在Cupriavidus sp.中增强多基酸盐的产量. 通过wcaJ基因删除L7L
Der-Shyan Sheu1, Ji-Long Chen1, Shih-Yi Sheu1
1Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan.
International journal of biological macromolecules
|October 17, 2023
概括
在Cupriavidus sp.中删除了wcaJ基因. L7L可以降低中等粘度和细胞外聚合物质. 这有助于更容易的细胞采集和增加聚酸 (PHA) 的生产,提高工业规模的效率.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
背景情况:
- 皮虫 (Cupriavidus sp.) 是一种的植物. 在L7L中,高含量的聚酸酸盐 (PHA) 产生.
- 在发酵过程中,高中等粘度限制了PHA生产和细胞采集.
- 细胞外聚合物物质 (EPS) 有助于增加粘度.
研究的目的:
- 调查wcaJ基因在EPS生产和中等粘度中的作用.
- 开发Cupriavidus sp.的一种非状变体. L7L用于改善发酵.
- 增强聚酸 (PHA) 的生产和下游加工.
主要方法:
- 使用迷你Tn5突变库对非粘膜变体的隔离.
- wcaJ的基因删除和补充实验.
- 野生类型和突变菌株的料批发发酵使用素酸盐 (Lev).
- 测量PHA含量,单体组成,EPS含量和中等粘度.
主要成果:
- 删除wcaJ消除了粘膜体殖民地形成,并显著降低了EPS含量和中等粘度.
- 皮虫 (Cupriavidus sp.) 是一种的植物. 在长时间发酵后,L7L∆wcaJ显示PHA度增加了42%.
- 通过离心机收集细胞对删除菌株来说明显更容易.
- 在删除菌株中,Lev-to-PHA的转化效率得到了提高.
结论:
- 在Cupriavidus sp.中,wcaJ基因对EPS的产生和高粘度至关重要. L7L. L7L. L7L. L7L. L7L. L7L. L7L. L7L. L7L. L7L.
- 皮虫 (Cupriavidus sp.) 是一种的植物. L7L∆wcaJ为高效和可扩展的聚酸 (PHA) 生产提供了一个有前途的平台.
- 减少EPS的基因改造是克服生物聚合物生产中的发酵挑战的可行策略.
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