塔莫西芬通过的积累和氧化应激诱导了红斑症
Mohammad A Alfhili1, Abdulaziz M Alyousef2, Jawaher Alsughayyir3
1Chair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 12372, Riyadh, Saudi Arabia. malfeehily@ksu.edu.sa.
Medical oncology (Northwood, London, England)
|October 17, 2023
概括
塔莫西芬 (TAM) 通过损害红细胞膜,导致贫血,从而导致红细胞死亡 (红细胞结核病). 维生素C,PEG 8000和尿素可能会防止TAM.
科学领域:
- 生物化学 生化学
- 血液学 血液学 血液学
- 毒理学 毒理学 毒理学
背景情况:
- 化疗诱导的贫血是癌症治疗中的一个重大挑战.
- 塔莫西芬 (TAM) 是一种抗雌激素药物,用于乳腺癌,但具有溶血潜力.
- 对TAM对红细胞 (RBC) 的侵蚀性影响尚未得到广泛研究.
研究的目的:
- 为了研究Tamoxifen (TAM) 对红细胞 (RBC) 的侵蚀性活性.
- 描述TAM诱导的红斑症背后的机制.
- 探索在红细胞中对TAM毒性的潜在保护剂.
主要方法:
- 人类红细胞被用不同度的TAM治疗.
- 测量了血红蛋白和乳酸脱酶 (LDH) 泄漏.
- 流式细胞计量评估了细胞膜的变化,的流入,细胞大小,氧化应激和亡标志物.
- 离子选择电极测量了细胞内离子度.
- 全血被用来评估对白细胞和血小板的毒性.
主要成果:
- TAM诱导剂量依赖的血液溶解和 (K+) 和AST的泄漏.
- TAM治疗导致附录素-V-FITC,Fluo4和DCF阳性增加,表明膜损伤,流入和氧化应激.
- 观察到细胞收缩 (FSC/SSC减少) 和乙胆酶 (AChE) 抑制.
- 糖糖加剧了TAM毒性,而维生素C,PEG 8000和尿素减轻了它.
- TAM对白细胞和血小板表现出明显的细胞毒性作用.
结论:
- 在红细胞中,TAM通过膜损伤,流入,细胞收缩和氧化应激诱导红细胞的 eryptosis.
- 维生素C,PEG 8000和尿素显示出对抗TAM诱导的红细胞毒性的潜力.
- 了解TAM的侵蚀性作用对于治疗癌症患者的贫血至关重要.
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