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开发一种针对纽卡斯尔病病毒的velogenic亚基因型七的疫苗,该疫苗基于类似病毒的颗粒
Masoumeh Firouzamandi1, Javad Ashrafi Helan2, Hassan Moeini3
1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. firouzamandi@gmail.com.
AMB Express
|October 17, 2023
概括
新型纽卡斯尔病病毒样颗粒 (NDV-VLPs) 诱导了强烈的T细胞反应,在中提供了完全的保护,防止velogenic NDV的挑战. 这突显了T细胞免疫力对B细胞反应对有效的NDV疫苗的重要性.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 疫苗开发 疫苗开发
背景情况:
- 纽卡斯尔病病毒 (NDV) 在全球对家禽构成重大威胁.
- 目前的疫苗可能无法完全保护高毒性NDV菌株.
- 了解对NDV免疫反应的细微差别对于开发改进的疫苗至关重要.
研究的目的:
- 开发和评估新型NDV病毒样颗粒 (NDV-VLP) 作为潜在的疫苗候选物.
- 为了比较NDV-VLP与传统B1活疫苗的免疫性和保护效果.
- 调查T细胞与B细胞反应在防护velogenic NDV中的作用.
主要方法:
- 使用M,HN和F糖蛋白生产和组装NDV-VLP.
- 用NDV-VLP对小鼠和进行免疫接种,有或没有B1活疫苗增剂.
- 使用ELISA (细胞因子,抗体) 和流细胞计 (CD8+ T细胞) 评估免疫反应.
- 在接种疫苗的中挑战使用毒性NDV亚基因型VII的研究.
主要成果:
- 在小鼠中,NDV-VLP显著增加IL2,IL10,TNF-α和IFN-γ细胞因子标位和CD8+T细胞计数.
- 虽然单独使用NDV-VLP与增强组相比,抗体标位没有显著增加,但在挑战后,它们在中提供了完整的生存.
- 仅用NDV-VLP接种疫苗的在病毒性NDV挑战后,比接受B1疫苗增剂 (66.7%) 的有更好的生存率 (100%).
结论:
- 与B细胞反应相比,T细胞介导的免疫在预防velogenic NDV方面起着更为关键的作用.
- NDV-VLP显示出作为疫苗平台的潜力,诱导强大的细胞免疫力.
- 针对velogenic NDV菌株的特定表位的疫苗是必要的,以便在中提供完整的保护.
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