单独的非同义突变在细胞染色体b高度与decoquinate耐药性高相关的寄生虫Eimeria tenella在apicomplexan
Zhenkai Hao1, Junmin Chen1, Pei Sun1
1National Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Parasites & vectors
|October 18, 2023
概括
细胞染色体b基因的突变是Eimeria tenella,一种家禽寄生虫中脱可酸耐药性的关键. 这些遗传变化可以作为分子标记物用于早期检测耐药性.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 埃梅里亚寄生虫导致的菌病,这是一个重要的家禽疾病.
- 抗杀菌药物耐药性,包括去可酸盐耐药性,对疾病控制构成了重大挑战.
- 需要分子标记物来监测Eimeria的耐药性.
研究的目的:
- 为了确定与Eimeria tenella.decoquinate耐药性相关的遗传突变.
- 评估这些突变作为药物耐药性检测分子标记物的潜力.
主要方法:
- 通过药物暴露开发出了耐德可酸盐的E. tenella菌株,并分离出了一种野外耐药菌株.
- 利用全基因组测序来识别耐药菌株中的突变.
- 采用分子建模来评估突变对德可酸结合的影响.
主要成果:
- 在所有抗德可酸盐耐药菌株中鉴定了细胞染色体b (cyt b) 基因的非同名突变.
- 观察到的突变 (Gln131Lys,Phe263Leu,Phe283Leu) 位于细胞b的Q extsubscript{o}结合部位附近.
- 分子对接表明突变的cyt b与decoquinate的结合亲和力降低.
结论:
- 在E. tenella cyt b基因中发生的突变对抗甲可酸耐药性至关重要.
- 基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因.
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