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R环充当调节开关,调节大米中COLD反应基因的转录

Zexue He¹, Mengqi Li¹, Xiucai Pan^1,2

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Collaborative Innovation Center for Modern Crop Production Co-Sponsored by Province and Ministry (CIC-MCP), Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu, 210095, China.

The New phytologist

|October 18, 2023

概括

寒冷压力严重影响到大米产量. 这项研究揭示了R环在寒冷压力期间如何重编程,调节基因表达,并为大米增强寒冷耐受性提供目标.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

寒冷压力是农业面临的主要挑战,导致农作物产量大幅下降.
R环是关键的核酸结构,参与植物发育和应激反应.
在大米的冷应激反应中R环的作用在很大程度上仍然未被描述.

研究的目的:

为了研究在寒冷压力下大米R环的全基因组重编程.
阐明R环影响大米冷反应基因表达的调节机制.
为了确定潜在的R-循环位点来改善大米的寒冷耐受性.

主要方法:

Drip-Seq (DNA-RNA免疫沉测序) 与RNA-Seq,DNase-Seq和ChIP-Seq一起使用.
在正常和寒冷条件下对R循环分布和相关基因组特征进行比较分析.
整合多omics数据以识别涉及R循环,转录因子和lncRNAs的监管网络.

主要成果:

冷处理诱导了大米基因组中显著的R循环重编程.
冷偏的R环呈现出更高的GC含量和独特的转录因子结合基因.
发现R循环通过调节网络调节约60%的冷诱导基因表达,与Arabidopsis的发现不同.
在冷反应基因表达中具有对立的调节作用的两个R环位点被确定并验证.

结论:

R循环重编程是大米冷应激反应的关键组成部分.
通过与转录因子和调节元件的相互作用,R环显著影响冷诱导的基因表达.
已识别的R-循环位点代表了基因和表观遗传工程的有希望的目标,以提高大米的寒冷耐受性.

关键词:

寒冷的反应反应.在DNase过敏位点.米树 (Oryza sativa) L. 米树 (Oryza sativa) L. 米树 (Oryza sativa) 是一个植物.在R-Loop中使用.基因转录基因转录在 lncRNAs 中.监管网络监管网络是指监管网络.

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