解读功能性长非编码RNAs来自微RNA位置的衍生
Weiqian Li1, Yue Huo1, Yue Ren1
1State Key Laboratory of Common Mechanism Research for Major Diseases, Institute of Basic Medical Sciences, Haihe Laboratory of Cell Ecosystem, The Key Laboratory of RNA and Hematopoietic Regulation, Chinese Academy of Medical Sciences / Peking Union Medical College, Beijing, 100005, P.R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 18, 2023
概括
研究人员发现了800多个miRNA基因起源的长非编码RNA (molncRNAs). 一个molncRNA,molnc-301b,调节基因表达和蛋白质翻译,影响红细胞形成.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞基因组被广泛转录,产生各种长的非编码RNA (lncRNAs).
- 微RNA (miRNA) 衍生 lncRNA 的全基因组研究是有限的.
- 了解ncRNA功能对于破译基因调节至关重要.
研究的目的:
- 为了对miRNA衍生 lncRNAs (molncRNAs) 进行全基因组研究.
- 为了描述molncRNAs在血液形成中的功能.
- 建立一个平台,对molncRNAs进行大规模的功能查.
主要方法:
- 从miRNA位置识别了800多个molncRNAs.
- 作为RNA诱的molnc-301b的功能性表征.
- 开发一个用于molncRNA功能分析的CRISPR选平台.
- 在血液细胞中对molncRNA功能的单细胞水平分析.
主要成果:
- 已经确定了800多个molncRNAs来自miRNA loci.
- molnc-301b作为RNA诱,将SMARCA5与染色质分离,抑制转录和翻译.
- molnc-301b通过降低关键的红色素质基因 (例如GATA1,FOS) 的调节来减弱红色素质形成.
- 一个CRISPR屏幕在造血细胞中发现了29个功能性molncRNA.
结论:
- miRNA衍生 lncRNAs 是一个重要的,以前研究不足的调节性RNAs 类.
- molncRNAs在调节基因表达,蛋白质翻译和血液形成等细胞过程中发挥着关键作用.
- 开发的CRISPR平台使molncRNAs的大规模功能基因组学成为可能.
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