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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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相关实验视频

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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

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解读功能性长非编码RNAs来自微RNA位置的衍生.

Weiqian Li1, Yue Huo1, Yue Ren1

  • 1State Key Laboratory of Common Mechanism Research for Major Diseases, Institute of Basic Medical Sciences, Haihe Laboratory of Cell Ecosystem, The Key Laboratory of RNA and Hematopoietic Regulation, Chinese Academy of Medical Sciences / Peking Union Medical College, Beijing, 100005, P.R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 18, 2023
PubMed
概括

研究人员发现了800多个miRNA基因起源的长非编码RNA (molncRNAs). 一个molncRNA,molnc-301b,调节基因表达和蛋白质翻译,影响红细胞形成.

关键词:
这是一个CRISPR屏幕.这就是SMARCA5的标志.在 miR-301b 里面.微RNA基因起源的 lncRNAs (molncRNAs) 是一种

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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相关实验视频

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09:39

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 细胞基因组被广泛转录,产生各种长的非编码RNA (lncRNAs).
  • 微RNA (miRNA) 衍生 lncRNA 的全基因组研究是有限的.
  • 了解ncRNA功能对于破译基因调节至关重要.

研究的目的:

  • 为了对miRNA衍生 lncRNAs (molncRNAs) 进行全基因组研究.
  • 为了描述molncRNAs在血液形成中的功能.
  • 建立一个平台,对molncRNAs进行大规模的功能查.

主要方法:

  • 从miRNA位置识别了800多个molncRNAs.
  • 作为RNA诱的molnc-301b的功能性表征.
  • 开发一个用于molncRNA功能分析的CRISPR选平台.
  • 在血液细胞中对molncRNA功能的单细胞水平分析.

主要成果:

  • 已经确定了800多个molncRNAs来自miRNA loci.
  • molnc-301b作为RNA诱,将SMARCA5与染色质分离,抑制转录和翻译.
  • molnc-301b通过降低关键的红色素质基因 (例如GATA1,FOS) 的调节来减弱红色素质形成.
  • 一个CRISPR屏幕在造血细胞中发现了29个功能性molncRNA.

结论:

  • miRNA衍生 lncRNAs 是一个重要的,以前研究不足的调节性RNAs 类.
  • molncRNAs在调节基因表达,蛋白质翻译和血液形成等细胞过程中发挥着关键作用.
  • 开发的CRISPR平台使molncRNAs的大规模功能基因组学成为可能.