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在大米中丰富的氨酸/氨酸 (SR) 基因家族的综合研究:特征,进化和表达分析分析

Rui Gao¹, Yingying Lu¹, Nan Wu¹

¹Department of Agronomy, The Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, China.

|October 18, 2023

概括

这项研究确定了大米中24个富含氨酸/氨酸 (SR) 的基因,通过全基因组分析和表达模式,揭示了它们在生长,发育和应激反应中的作用.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
基因规则基因规则

背景情况:

富含氨酸/氨酸 (SR) 的蛋白质是生物体中替代拼接 (AS) 的关键调节者.
在植物,特别是大米中,SR基因家族研究尚未得到充分发展.

研究的目的:

对大米 (Oryza sativa) 中的SR基因家族进行全面的全基因组分析.
研究米SR基因的遗传学关系,基因结构,保存域和进化扩张.
探索大米SR基因在发育和应激反应中的表达模式和潜在功能.

主要方法:

在大米中对SR基因进行全基因组分析.
遗传学分类和合成分析 (包括跨种类比较).
对替代拼接事件,组织特异性表达和促进者cis元素分布的分析.
在各种压力和激素治疗 (ABA,GA,盐,干旱,寒冷,热量) 下基因表达概况.

主要成果:

在大米中确定了24个OsSR基因,根据遗传学分析将其分为七个子家族.
在子家族中保存了基因结构,域和蛋白质三级结构.
分段重复是OSSR基因家族扩张的关键驱动力;与其他草种建立了正义关系.
对于OSSR基因,观察到不同的组织特异性表达模式和可变的AS事件.
一些OSSR基因在非生物压力和激素治疗下显著改变的表达,与促进者cis元素相关.

结论:

米中的OsSR基因家族在结构上是保存的,并且通过重复扩大了.
OsSR基因在米发育,组织分化和对环境压力的反应中发挥着不同的作用.
这项研究为了解SR基因功能及其在大米中的调节提供了基础.

关键词:

无生物应激应激是一种非生物应激.另一个替代拼接.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式在OSSR基因的基因.米 (Oryza sativa L.) 是一种大米.

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