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使用基于过器的DNA丰富方法和纳米孔测序获得的"Candidatus Phytoplasma sacchari"的完整基因组序列

Rong-Yue Zhang^1,2, Xiao-Yan Wang¹, Jie Li¹

¹Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan, China.

Frontiers in microbiology

|October 18, 2023

概括

一种新的DNA丰富方法显著改善了甘中的植物质检测. 这项研究为"Candidatus Phytoplasma sacchari"提供了第一个完整的基因组序列,有助于对甘疾病的研究.

科学领域:

植物病理学植物病理学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

植物质像甘白叶 (SCWL) 植物质一样,对甘作物造成重大经济损害.
在体外培养植物质体具有挑战性,限制了它们的表征.
基因组测序为研究植物质生物学提供了新的途径.

研究的目的:

开发和评估一种用于SCWL植物质的DNA丰富方法.
为了获得"Candidatus Phytoplasma sacchari"的完整基因组序列,使用Illumina和Nanopore测序分离SCWL1.
分析SCWL1分离的基因组特征和遗传学关系.

主要方法:

开发一种用于SCWL植物质的DNA丰富技术.
应用Illumina和纳米孔测序技术用于基因组组装.
生物信息分析包括读取映射,基因识别,遗传学分析,ANI和dDDH.

主要成果:

通过DNA丰富方法,SCWL1基因组读取映射从1.21%增加到40.97%.
"Ca. 的完整基因组. 植物质糖体SCWL1,包括一个染色体和一个质粒,被测序.
遗传学和基因组分析表明,SCWL植物质和甘草草芽植物质是同一物种.

结论:

开发的DNA丰富方法对植物质体测序是有效的.
这项研究提供了第一个完整的基因组序列"Ca. 植物等离子体糖分离子.
这些发现有助于更好地了解植物质细胞的进化和病原性,特别是对于甘疾病.

关键词:

通过DNA丰富进行DNA丰富.基因组测序是指对基因组进行测序.植物等离子体的植物质.甘的草生长甘的草生长甘的白色叶子甘的白叶子

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