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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

23
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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相关实验视频

Updated: Jul 13, 2025

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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持久的基于CRISPR的表观遗传沉默

Muneaki Nakamura1, Alexis E Ivec1,2, Yuchen Gao1,3

  • 1Department of Bioengineering, Stanford, CA 94305USA.

Biodesign research
|October 18, 2023
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种CRISPR表观基因组编辑记者系统,以测量稳定的基因抑制. 该工具有助于为生物研究和工程应用创建新的表观基因组编辑技术.

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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因编辑技术的技术

背景情况:

  • 基于CRISPR的表观基因组编辑对于理解和操纵生物功能至关重要.
  • 开发精确控制基因表达的工具对于生物研究至关重要.

研究的目的:

  • 设计和验证一个报告员系统,以量化CRISPR表观基因组编辑器在实现稳定基因抑制方面的有效性.
  • 用CRISPR表观基因组编辑工具来描述基因沉默和重新激活的动态.

主要方法:

  • 开发一个报告系统来评估CRISPR表观基因组编辑器的性能.
  • 创建包含表观遗传编辑域 (称为KAL) 的单蛋白CRISPR结构.
  • 基因沉默,重新激活动态和相关的表观遗传修饰的特征.

主要成果:

  • 成功设计了一种用于量化稳定基因抑制的记者系统.
  • 单蛋白CRISPR结构 (KAL) 能够进行持久的基因沉默的演示.
  • 描述基因沉默和重新激活的动态,以及诱导的表观遗传变化.

结论:

  • 开发的报告员系统可以评估CRISPR表观基因组编辑工具,以实现稳定的基因抑制.
  • 卡尔结构为新型表观基因组编辑工具提供了基础.
  • 这项工作促进了表观基因组编辑的进步,用于各种生物研究和工程应用.