ERC-BiP功能性蛋白质途径用于评估细胞入侵后由SARS-CoV-2复制引起的内分泌网膜压力
Mingshan Xue1,2,3, Zhiwei Lin3, Teng Zhang4,5
1Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, China.
概括
SARS-CoV-2 感染会引起细胞内膜网膜 (ER) 压力,通过ERC-BiP,AGT和Cer水平升高以及SM降低来识别. 这种蛋白质代谢途径有助于预测COVID-19的严重程度.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- SARS-CoV-2 侵入细胞,诱导细胞亡和通过细胞内网膜 (ER) 应激通过免疫反应.
- 了解与ER功能障碍相关的蛋白质代谢途径对于COVID-19研究至关重要.
研究的目的:
- 在SARS-CoV-2感染中建立与ER功能障碍相关的蛋白质代谢途径.
- 确定用于预测COVID-19进展的生物标志物.
主要方法:
- 从17名健康个体和46名COVID-19患者 (38名轻度,8名重度) 的血蛋白质组学和代谢学分析.
- 根据一周内疾病进展情况,患者被分为恶化和缓解组.
- 进行了纵向和横截面分析,以比较生物标志物水平.
主要成果:
- 与健康对照人群相比,COVID-19患者的ERC-BiP,AGT,Cer和CRP水平显著增加,SM降低.
- 病情恶化的患者比缓解期患者有更高的ERC-BiP,AGT和Cer,以及较低的SM水平.
- ERC-BiP与AGT有显著的相关性.
结论:
- ERC-BiP作为COVID-19患者ER压力的关键指标,对评估细胞功能有价值.
- AGT/ERC-BiP/糖解通路可以评估未折叠的蛋白质反应.
- 由于ERC-BiP途径与SARS-CoV-2复制很接近,这表明它有可能在开发COVID-19恶化预测协议方面发挥作用.
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