在小鼠中,丁受体在三角细胞控制β细胞功能的过程中没有作用
Jia Zhang1, Kay Katada2, Elham Mosleh1,2
1Department of Genetics, University of Pennsylvania, Philadephia, PA, United States.
Frontiers in endocrinology
|October 18, 2023
概括
勒普丁是一种激素.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 代谢研究研究 代谢研究
背景情况:
- 莱普对胰岛素分泌的抑制已被证实,但介导这种作用的特定岛屿细胞类型尚不清楚.
- 之前的研究表明,胰腺上皮质中的素受体 (Lepr) 表达,但不仅仅是在α或β细胞中.
- 人类小岛单细胞RNA测序数据表明LEPR在三角洲细胞中的丰富.
研究的目的:
- 为了研究胰腺三角细胞是否调解瘦素对小鼠胰岛素分泌的抑制作用.
- 确认人类三角细胞中的LEPR表达,并评估其在葡萄糖平衡中的功能作用.
主要方法:
- 分类人类β和delta细胞的RNA测序,以确认LEPR表达.
- 产生和分析一只小鼠模型的勒普拉切除,特别是在三角细胞.
- 在小鼠吃和高脂肪饮食中评估葡萄糖平衡和胰岛素分泌.
- 对公开可用的单细胞RNA测序数据进行分析,以识别小岛上的表达勒布细胞.
主要成果:
- 在正常或高脂肪饮食条件下,在小鼠三角细胞中切除Lepr并没有改变葡萄糖平衡或胰岛素分泌.
- RNA测序证实了人类三角细胞中的LEPR上调.
- 对scRNAseq数据的分析揭示了Lepr在内皮细胞集群中的表达,而不仅仅是三角形细胞.
结论:
- 在小鼠中,瘦素不会通过三角细胞对β细胞功能产生影响.
- 负责瘦素对小鼠胰岛素分泌的作用的细胞类型尚未确定,可能涉及内皮细胞.
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