使用植物uORF来控制对病原体攻击的响应中转基因翻译
Gan Ai1, Jin Liu1, Xiaowei Fu1
1College of Plant Protection, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.
Biodesign research
|October 18, 2023
概括
在作物中,可以使用上游开放式读取框架 (uORFs) 来提高工程疾病耐药性. 这些uORF精细调整基因转换,增强作物对病原体的保护,如Phytophthora capsici.
科学领域:
- 植物生物技术 植物生物技术
- 分子遗传学 分子遗传学
- 农作物科学 农作物科学
背景情况:
- 全球粮食安全受到农作物疾病的威胁,人口增长和气候变化加剧了这种威胁.
- 转基因方法来增强作物抗病能力可能会导致不良的发育效应.
- 在mRNA的5'未翻译区域的上游开放读取框架 (uORF) 调节基因翻译,并可能提供解决方案.
研究的目的:
- 为了研究加速细胞11 (uORFsACD11) 的5'-UTR中的三个uORF的功能.
- 确定uORFsACD11是否可以用于增强植物抗病能力.
- 探索uORFsACD11作为控制植物蛋白表达的工具包的潜力.
主要方法:
- 开发一个光报道系统来研究uORF的功能.
- 单个和多个uORFsACD11.11的局部定向突变发生.
- 在正常条件和病原体攻击条件下对转化效率的分析.
- 克隆uORFsACD11录音带,其中含有与病原发育相关的基因1 (PR1) 促剂.
主要成果:
- uORFsACD11被证实可以抑制下游基因翻译.
- uORFsACD11中的突变对转化抑制有不同的影响.
- 在病原体识别时,uORFsACD11的转化抑制减少.
- 在使用uORFsACD11与PR1促进剂时,观察到Arabidopsis thalianaLECTIN受体KINASE-VI.2 (AtLecRK-VI.2) 的升级和对Phytophthora capsici的增强耐药性.
结论:
- uORFsACD11 作为翻译抑制剂.
- uORFsACD11系统允许诱导性蛋白质表达,在病原体攻击期间增加.
- 本工具包提供了一种提高作物抗病能力的策略,而不会影响正常条件下的生长.
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