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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 13, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
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对具有复杂细结构的悬浮无标签淋巴细胞进行散射反转研究.

Lu Zhang1, Huijun Wang1, Jianyi Liu2

  • 1Xi'an Jiaotong University, School of Mechanical Engineering, State Key Laboratory for Manufacturing Systems Engineering, Xi'an 710049, China.

BME frontiers
|October 18, 2023
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概括

这项研究引入了反光散射 (ILS) 方法来测量淋巴细胞体积,有助于病理诊断. 该技术可以准确地区分恶性细胞和正常细胞,而不会损害样本.

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科学领域:

  • 生物物理学的生物物理.
  • 病理学 病理学 病理学
  • 光学方法 光学方法.

背景情况:

  • 光散射提供了与生物物理状态相关的细胞形态信息.
  • 了解复杂的细胞结构和分散特征之间的关系对于病理学分析至关重要.

研究的目的:

  • 开发和验证一种反光散射 (ILS) 方法,用于对悬浮淋巴细胞体积进行无标签的定量分析.
  • 通过根据淋巴细胞的形态来区分恶性淋巴细胞和正常淋巴细胞来确定病态状态.

主要方法:

  • 模型临床淋巴细胞形态使用高斯随机球体几何和共聚焦扫描数据.
  • 开发了一种ILS方法,以定量逆转细胞膜和细胞核的体积.
  • 研究了ILS对复杂结构的特异性,包括表面粗度,姿势和折射率.

主要成果:

  • 证明了膜/核体积和前向散射图像特性 (有效面积和) 之间的线性关系.
  • 在ILS方法中实现的特异性偏差低于3.5%.
  • 用微球和临床白细胞验证了该方法,显示了高的皮尔森产品时刻相关系数 (PPMCC),高达0.9926.

结论:

  • 拟议的ILS方法有效地识别悬浮的,无标签的淋巴细胞.
  • 这种技术提供了一种非破坏性的方法,用于在没有复杂的实验设置的情况下进行病理检查.