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灵敏地检测人体血清和心肌细胞中的内源性homocysteine,使用特定的光探针探测

Huan Li¹, Qiwei Wang¹, Lili Shi²

¹Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China. tlitao@ustc.edu.cn.

|October 18, 2023

概括

高水平的同氨酸 (Hcy) 是急性心肌梗塞 (AMI) 的风险因素,但这项研究表明循环中的Hcy水平与心肌细胞损伤没有直接相关,因此不适合用于AMI诊断.

科学领域:

生物化学生物化学
医学诊断医学诊断医学诊断
光探测器光探测器

背景情况:

高水平的同类氨酸 (Hcy) 是急性心肌梗塞 (AMI) 的已知危险因素.
对于AMI的循环Hcy水平的诊断实用性仍然不清楚.
像氨酸 (Cys) 和谷氨酸 (GSH) 这样的生物醇可以干扰Hcy检测.

研究的目的:

开发一种选择性光探针,用于在生物样本中检测敏感的Hcy.
调查Hcy水平和AMI之间的关系.
为了确定Hcy是否可以作为AMI诊断的可靠生物标志物.

主要方法:

基于四次循环的光探针的设计和合成.
在AMI患者和健康个体的血清和心肌细胞中检测内源性Hcy.
对Hcy水平的统计分析和与其他生物乙醇的比较.
评估心肌细胞与其他组织细胞中的Hcy表达.

主要成果:

开发的探测器对Cys和GSH检测Hcy具有很高的灵敏度和选择性.
在AMI患者和健康参与者之间观察到循环Hcy水平的显著差异.
与其他组织细胞相比,心肌细胞中的内源性Hcy水平较低.
在高循环Hcy和心肌细胞损伤之间没有直接的相关性.

结论:

光探针可以有效地检测生物流体中的Hcy.
尽管是风险因素,但循环中的Hcy水平并不是心肌细胞损伤的直接指标.
循环中的Hcy不能被可靠地用作AMI诊断的生物标志物.

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