脂蛋白纳米颗粒的组成和大小对它们在植物和哺乳动物细胞内的内化的影响
Ryosuke Fukuda1,2, Misaki Tani2,3, Shiori Shibukawa2,3
1Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Toyama, Japan.
Genes to cells : devoted to molecular & cellular mechanisms
|October 18, 2023
概括
工程脂蛋白 (eLPs) 在植物和哺乳动物细胞中显示不同的细胞吸收. 脂质组成和粒子特性显著影响eLP内部化,为向药物递送系统提供了洞察力.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 纳米医学是一种纳米医学.
背景情况:
- 工程高密度脂蛋白纳米颗粒 (eLPs) 对药物输送有希望.
- 了解细胞内部化机制对于优化纳米粒子设计至关重要.
研究的目的:
- 在植物 (BY-2) 和哺乳动物 (HeLa) 细胞中分析不同组成的ELP内部化.
- 研究脂质组成,大小,泽塔潜力和细胞透对ELP吸收的影响.
- 探索跨王国药物交付中的潜在应用.
主要方法:
- 合成的ELP具有多样化的脂质和蛋白质组成.
- 不同的ELP大小和泽塔潜力.
- 使用光显微镜评估了BY-2原生体和HeLa细胞中的ELP内部化.
- 研究了一种细胞透对ELP吸收的影响.
- 检查了二甲酸二甲糖醇 (DGDG) 对ELP和脂质体内化的影响.
主要成果:
- 与HeLa细胞相比,细胞透对BY-2原生细胞吸收的影响很小.
- 结合DGDG的eLP在HeLa细胞中显著增加了内部化,但不是BY-2原体质.
- 脂质体中的DGDG含量与增加的HeLa细胞吸收相关.
- 更大的尺寸和更高的泽塔潜力通常在两种细胞类型中增强了ELP内部化,但程度不同.
- 在BY-2原生体的血中积累的eLP,促进共化纳米珠的吸收.
结论:
- eLPs的细胞吸收高度依赖脂质组成和颗粒特征,植物和哺乳动物细胞之间存在显著差异.
- 独特的内部化途径突出挑战和跨王国纳米医学的机会.
- 研究结果为设计针对性脂质纳米粒子用于各种生物系统提供了基本的见解.
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