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一种Staphylococcus capitis菌株具有不寻常的细菌素生产

Christiane Szekat¹, Michaele Josten¹, Jasmin Rickmeyer¹

¹Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.

Microbial biotechnology

|October 18, 2023

概括

斯塔菲洛科克斯卡皮蒂斯菌株BN2产生新型细菌素和可溶于的模块,在体外有效抑制耐甲基西林黄金葡萄球菌 (MRSA) 和菌素中间体黄金葡萄球菌 (VISA). 这一发现突出了对抗抗生素耐药细菌的潜在新途径.

科学领域:

微生物学微生物学
细菌学细菌学是一门学科.
基因组学就是基因组学.

背景情况:

斯塔菲洛科克斯 (Staphylococcus capitis) 是一种共生性皮肤细菌,通常被认为比金黄色葡萄球菌 (Staphylococcus aureus) 致病率低.
抗生素耐药的菌株,如耐甲基黄金葡萄球菌 (MRSA) 和菌素中间体黄金葡萄球菌 (VISA),对公共卫生构成重大挑战.
VISA菌株的细菌素耐药性通常与细胞壁和膜的改变有关.

研究的目的:

为了研究Staphylococcus capitis亚种的抗菌性质. 尿素分析器 BN2.2.
为了确定对抗性黄金葡萄球菌菌株抗菌活性负责的化合物.
描述抗菌物质生产的遗传基础.

主要方法:

隔离和培养斯塔菲洛科科克斯 (Staphylococcus capitis) 亚种. urealyticus BN2 来自猫类来源的.
基因组测序用于识别抗微生物物质生物合成的基因集群.
使用质谱学分析细胞提取物和整个细胞.
基因操纵 (删除NRPS模块) 来评估特定化合物的作用.
在体外对抗MRSA和VISA菌株的竞争测试.

主要成果:

斯塔菲洛科克斯 (Staphylococcus capitis) 的一个亚种. urealyticus BN2对包括MRSA和VISA在内的格拉姆阳性细菌表现出强大的抗菌活性.
基因组分析揭示了利德明兰西生物的基因集群和一种新的非核糖体合成酶 (NRPS).
一种由装饰的非核糖体组成的新型细菌素被确定为一种关键的抗VISA药物.
还检测到溶性模块 (PSM),可能导致竞争排斥.
这种菌株在体外成功超越了MRSA和VISA.

结论:

斯塔菲洛科克斯 (Staphylococcus capitis) 的一个亚种. urealyticus BN2产生了一种抗微生物化合物的组合,包括一种新型细菌素和PSM.
这些化合物赋予了与临床相关的抗生素耐药性黄金葡萄球菌菌株的竞争优势.
这些发现表明,基于这些抗微生物物质,有可能开发新的治疗策略.

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