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生殖线能力的多个维度

Seth Teague¹, LiAng Yao², Idse Heemskerk³

¹Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.

Current opinion in cell biology

|October 18, 2023

概括

人类多能干细胞 (hPSCs) 对原始生殖细胞样细胞 (PGCLC) 差异化表现出高生殖细胞能力. 局部细胞相互作用,而不是独特的状态,影响PGCLC的发展,表明细胞状态的复杂景观.

科学领域:

发育生物学发展生物学
干细胞生物学干细胞生物学
生殖生物学生殖生物学

背景情况:

原始生殖细胞 (PGC) 规范对于生殖细胞发展至关重要.
人类和小鼠的PGC分化表现出差异,在实验室中确定了PGC样细胞 (PGCLC) 分化的细胞状态.
在体外PGCLC能力状态,胚胎发育和体内PGC起源之间的关系仍在争论中.

研究的目的:

在与小鼠和非人类灵长类动物模型相比,审查人类PGCLC差异化文献.
重新评估人类多能干细胞 (hPSCs) 的生殖能力.
解释hPGCLC起源和信号要求中的悖论性观察.

主要方法:

对人类,小鼠和非人类灵长类动物PGCLC分化研究的文献综述.
在体外细胞间相互作用和信号梯度的分析.
发展胚胎能力的概念框架.

主要成果:

与之前的假设相反,人类多能干细胞 (hPSCs) 显示出显著的生殖能力.
局部细胞相互作用产生内源信号梯度,混PGCLC分化试验.
单一的,独特的生殖系有能力状态不太可能;在更高维度的细胞状态景观中存在能力.

结论:

在hPGCLC发育中的悖论性发现可能源于局部细胞环境和信号传递.
胚芽能力不是一个线性光谱,而是细胞状态的复杂,多维的景观.
考虑到细胞相互作用,重新审视PGC发育模型是必不可少的.

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