基于细胞表的in vitro骨缺陷模型,用于长期评估骨修复材料
Jing Gao1,2,3, Yuqi Li4, Tingting Wu1,2,3
1Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, People's Republic of China.
Biomedical materials (Bristol, England)
|October 18, 2023
概括
使用人类干细胞表的新型3D骨缺陷模型有效评估骨修复材料 (BRM). 这种模型显示了准确,快速评估BRM骨质诱导性的前景,减少了对动物试验的依赖.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 开发可靠的实验室模型来评估骨修复材料 (BRM) 对于解决与动物模型相关的伦理和翻译问题至关重要.
- 人类骨髓中介质干细胞片可以被改造成类似骨周的组织,以创建先进的*体外*骨缺陷模型.
研究的目的:
- 开发和验证一种新的三维 (3D) 组织工程人类骨缺陷模型,用于评估BRM的细胞毒性和骨诱导性.
- 为了比较3D模型的性能与2D培养模型和BRM评估的"in vivo"实验.
主要方法:
- 人类骨髓介质干细胞被培养成细胞片并堆叠在一起,以创建一个3D骨周状结构.
- 在3D模型缺陷中植入了三种商业BRM,并使用CCK-8测定,西部斑,RT-qPCR和阿里扎林红色染色来评估它们的影响.
- 使用2D培养模型和*in vivo*动物实验进行了比较研究.
主要成果:
- 与2D模型相比,3D模型对BRM细胞毒性表现出优异的耐药性,与*in vivo*发现保持一致.
- 在3D模型中植入BRM诱导了关键骨质原生标志物 (RUNX2,OSX,OCN,OPN) 的显著上调.
- 材料B和C表现出比材料A更强的骨质诱导性,证实了矿化结节的形成和基因表达的增加,可能与离子释放有关.
结论:
- 开发的基于细胞板的3D骨组织模型准确地预测了BRM骨质诱导性,与*体内*结果良好相关.
- 该模型为快速准确评估BRM提供了一个有希望的替代方案,有可能加速其研发过程.
- 该模型能够评估长期的骨质诱导性和抵抗细胞毒性,这突显了其作为再生医学研究中宝贵工具的潜力.
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