相关实验视频

Updated: Jul 13, 2025

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Published on: September 5, 2013

AGPATs 异型的表达模式表明,在中国大黄,Eriocheir sinensis 中的三糖化物合成过程中,它们的功能不同

Kewu Pan¹, Boxiang Zhu¹, Lin Wang¹

¹Shanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

|October 18, 2023

概括

三种1-乙甘-3-酸盐乙转移酶 (AGPAT) 异型被鉴定出在中国大白中. 它们独特的表达模式表明它们在化和卵巢发育期间的三糖醇 (TAG) 合成中发挥着独特的作用.

科学领域:

生物化学生化学
分子生物学分子生物学
甲动物生物学甲动物生物学

背景情况:

三糖醇 (TAG) 合成对于许多生物体的能量储存至关重要.
一甲基甘三酸盐乙转移酶 (AGPAT) 酶是TAG合成的关键调节者.
了解AGPAT异型函数对于阐明代谢途径至关重要.

研究的目的:

为了识别和表征在中国小,Eriocheir sinensis.的AGPAT异型.
为了研究Es-agpat异型体在脱皮和卵巢发育期间的表达模式.
探索Es-agpat异型在三糖醇合成中的作用.

主要方法:

在E. sinensis中鉴定了三种AGPAT异型 (Es-agpat2,Es-agpat3,Es-agpat4).
在各种组织和发育阶段对mRNA表达水平的定量分析.
在现场杂交 (ISH) 以确定组织特定的局部化.
基因沉默实验用于评估功能角色.

主要成果:

埃斯-阿格帕特2和埃斯-阿格帕特4在肝胰腺,心脏,肌肉和胸腺质中表达高,在脱皮和卵巢发育期间具有特定的模式.
埃斯-阿格帕特3在胸腺节中丰富,并显示出明显的卵巢表达.
在发育过程中,ISH在特定的肝细胞中发现了Es-agpat4,在卵巢组织中发现了Es-agpat3/4.
沉默Es-agpat2和Es-agpat4导致下游TAG合成基因的表达减少.

结论:

三种已识别的Es-agpat异型 (Es-agpat2,Es-agpat3,Es-agpat4) 可能在E. sinensis中具有不同的功能.
这些异构体在化周期和卵巢成熟期间在三糖醇合成中发挥着重要作用.
进一步的研究可以阐明这些AGPATs在脂质代谢中的精确分子机制.

关键词:

AGPAT的同位素是AGPAT的同位素.这里是Eriocheir sinensis的原点.脂质代谢脂质代谢是一种化化化卵巢发育卵巢发育这是一种RNA干扰.

更多相关视频

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Published on: June 25, 2013

Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR

Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR

Published on: May 4, 2010

相关概念视频

GPI Anchoring of Proteins in the ER Membrane

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...

Synthesis of Phosphatidylcholine in the ER Membrane

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...