电化学H2O2 - 状态模式作为反应概念,以提高不特定的过氧酶的过程性能
Giovanni V Sayoga1, Victoria S Bueschler1, Hubert Beisch2
1Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestraße 15, 21073 Hamburg, Germany.
New biotechnology
|October 18, 2023
概括
本研究比较了使用Agrocybe aegerita非特异性过氧化酶 (rAaeUPO) 在电酶化化中提供过氧化的两种方法. 保持恒定的H2O2度的H2O2-stat模式显著提高了酶的性能和生产力.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 电化学和电催化学 电催化学
- 绿色化学和可持续的过程
背景情况:
- 非特异性过氧酶 (UPO) 是氧化反应的多功能酶.
- 电酶系统中的酶稳定性和生产率对过氧化 (H2O2) 供应敏感.
- 优化H2O2输送对于高效的UPO催化过程至关重要.
研究的目的:
- 为了比较两个in-situ H2O2电生成模式,用于UPO催化电酶氧化.
- 评估静电和H2O2-stat模式对酶性能和生产力的影响.
- 确定提高UPO流程效率的最佳条件.
主要方法:
- 使用重组Agrocybe aegerita非特异性过氧酶 (rAaeUPO) 的4-乙基酸的电酶氧化.
- 基于气体扩散电极 (GDE) 的系统用于现场的H2O2发电.
- 静电 (恒定的H2O2生产率) 和H2O2-stat (恒定的H2O2度) 模式的比较.
主要成果:
- 静电模式实现了最大的H2O2生产率为5.5μMmin-1cm-2和10.5gL-1d-1在6.4mAcm-2.4时.
- H2O2-stat模式产生了高达710,000 mol mol-1的总周转数 (TTN) 和高达87.5 s-1.1的周转频率 (TOF).
- 在H2O2-stat模式下,在0.2mM H2O2度下观察到最佳性能 (TTN,TOF,生产率).
结论:
- 电化学H2O2-stat模式是UPO应用中对静电模式的有希望的替代方案.
- H2O2-stat模式显著提高了酶稳定性,周转率和整体工艺生产率.
- 这种优化的电酶方法促进了过氧酶在生物催化剂中的应用.
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