规模和检测方法影响了池中的沙门氏菌流行率和多样性
Claire M Murphy1, Daniel L Weller2, Laura K Strawn1
1Department of Food Science and Technology, Virginia Tech, 1230 Washington Street SW, Blacksburg, VA 24061, USA.
The Science of the total environment
|October 18, 2023
概括
了解食物传播病原体生态对于农业水安全至关重要. 这项研究发现,弗吉尼亚州池中的沙门氏菌污染因检测方法和环境因素而异,强调需要个性化的池管理.
科学领域:
- 环境微生物学 环境微生物学
- 食品安全科学 食品安全科学
- 农业用水质量 农业用水质量
背景情况:
- 农业中有效的食品安全管理需要了解水源中的食物传播病原体生态.
- 针对特定地点的战略对于减轻农业用水系统中的危险至关重要.
研究的目的:
- 为了确定与弗吉尼亚州农场池中的沙门氏菌污染相关的因素.
- 为了比较基于培养和培养独立 (CIDT) 方法之间的沙门氏菌检测率.
- 评估环境变量对农业用水中的沙门氏菌存在的影响.
主要方法:
- 从30个地点收集了600个抓取样本,分布在维吉尼亚州的9个池.
- 利用基于培养和CIDT的方法来检测沙门氏菌.
- 使用考夫曼-怀特分类的血清型培养沙门氏菌分离物.
- 收集环境数据并使用混合效应模型来分析关联.
主要成果:
- 使用CIDT (31%) 的沙门氏菌检测率明显高于基于培养的方法 (13%).
- 培养方法确定了11种沙门氏菌血清;42.1%的CIDT阳性样本是培养阳性的.
- 环境因素,如总大肠杆菌,大肠杆菌,温度,紫外线和雨水影响了沙门氏菌的检测,雨水增加了检测几率.
- 环境因素和沙门氏菌之间的关联在个别池之间有很大差异.
结论:
- 沙门氏菌检测方法和分析规模 (单个池与组合) 显著影响观察到的水质.
- 个性化水资源管理计划至关重要,因为池特定的因素会影响沙门氏菌污染.
- 这些发现强调了在农业用水监测计划中考虑检测方法和特定地点风险的重要性.
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