通过从水果废物中使用酸原衍生H2和CO2进行聚氨酸酸盐的自营生产
Paolo Costa1, Marina Basaglia2, Sergio Casella2
1Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), Waste to Bioproducts-Lab, Università di Padova, Agripolis, Viale dell'Università 16, Legnaro, Padua 35020, Italy; Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of A Coruña, Rúa da Fraga 10, Coruña 15008 A, Spain.
Bioresource technology
|October 18, 2023
概括
果物废弃物的利用产生生物塑料! 这项研究将瓜子废物转化为和二氧化碳,然后使用这些气体生产聚酸酸盐 (PHAs),一种可持续的塑料替代品.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 化石塑料污染需要可持续的替代品,如聚酸酸盐 (PHAs).
- 高昂的生产成本目前限制了PHA的广泛采用.
- 使用CO2和H2的PHAs的自营微生物生产提供了一个有希望的途径,但通常依赖于昂贵或有毒的气体来源.
研究的目的:
- 调查从水果废弃物发酵中获得的CO2和H2中生产PHA的可行性.
- 为了证明酸原性衍生气体的自转化为聚3-基酸盐 (P(3HB)) 和P(3HB-co-3HV) 共聚合物.
主要方法:
- 采用了两阶段的生物工艺:首先,通过混合微生物群体对西瓜废物进行酸性发酵,以产生H2和CO2.
- 其次,Cupriavidus necator DSM 545在生物反应器中进行自培养,使用生成的H2和CO2进行PHA积累.
- 分析了用于共聚合物生产的酸性生成过程中产生的挥发性脂肪酸 (VFA).
主要成果:
- 通过使用自性C. necator养H2 (26.7%) 和CO2 (49.2%) 的瓜子废物,成功产生了1.7g/L的P(3HB).
- 此外,从初始酸性形成阶段产生的VFA中产生了2.7g/L的P(3HB-co-3HV).
- 这标志着PHA生产的首次演示,使用来自水果废弃物酸性发酵的H2和CO2.
结论:
- 通过两阶段的发酵过程,水果废弃物可以有效地被提升为有价值的生物塑料 (PHAs).
- 这种方法为PHA生产提供了一种具有成本效益和可持续性的方法,利用废物流并减少对化石燃料的依赖.
- 这项研究为生物聚合物合成农业废物的综合生物处理提供了一个新的概念验证.
相关概念视频
Microbial Fermentation
35
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
35
Carbon-dioxide Fixation
19
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
19
Fates of Pyruvate
8.5K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.5K
Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis
2.9K
Hydrolysis of esters under acidic conditions proceeds through a nucleophilic acyl substitution. In the presence of excess water, the reaction proceeds in a reversible manner, forming carboxylic acids and alcohols.
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
2.9K
Products of the Citric Acid Cycle
99.0K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
99.0K
Bioremediation
18.7K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.7K


