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Updated: Jul 13, 2025

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

建议通过in situ杂交来区分HER2-低和HER2-正常的乳腺癌

Yen-Ying Chen^1,2,3, Ching-Fen Yang^3,4, Chih-Yi Hsu^5,4,6

¹Department of Pathology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan.

Journal of clinical pathology

|October 18, 2023

概括

识别低HER2乳腺癌对于向治疗至关重要. 目前的免疫组织化学 (IHC) 方法是不够的;使用良性腺体参考值的现场杂交 (ISH) 为准确的HER2-低检测提供了一个有希望的替代方案.

科学领域:

在瘤学瘤学.
分子病理学分子病理学
乳腺癌研究研究乳腺癌研究

背景情况:

"HER2-低"乳腺癌是一种新兴的分类,对特拉斯图祖马布 (trastuzumab) 等向治疗具有重要意义.
准确识别HER2-低状态对于临床试验中的患者选择和治疗决策至关重要.
现有的免疫组织化学 (IHC) 方法在可靠区分HER2-低病例方面面临挑战.

研究的目的:

评估免疫组织化学 (IHC) 和现场杂交 (ISH) 检测低HER2乳腺癌的疗效.
使用ISH比较IHC得分0和1+瘤之间的HER2表达水平.
确定周周瘤良性腺体中HER2 ISH的参考值,以准确评估HER2低.

主要方法:

来自能力测试 (PT) 的HER2 ISH数据基于IHC共识进行了分析.
内部组织微阵列 (TMA) 用于比较IHC 0和IHC 1+瘤中的HER2 ISH结果.
围瘤良性腺体中的HER2 ISH被评估为参考标准.

主要成果:

没有IHC共识在PT中达成80%的IHC-1+协议.
与IHC-0核相比,IHC-1+核中的HER2信号显著高于IHC-0核,但具有相当大的重叠.
在良性腺体中确定了HER2信号和HER2:CEP17比率的95%参考区间的上限.

结论:

目前的HER2测试算法不足以准确识别低HER2病例.
使用ISH来识别超出良性腺参考值的HER2信号和HER2:CEP17比率的瘤是一个可行的替代方案.
这种基于ISH的方法为 HER2-低乳腺癌检测提供了更高的准确性.

关键词:

乳腺新生体乳腺新生体这些基因, erbB-2 在现场混合化.染色和标签的使用

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In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...