一种P450-单氧基酶可实现Epoxycyclohexenones的区域和立体选择性生物催化合成
Yan-Long Yang1,2, Man Zhou1, Lin Yang1
1State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, 730000, Lanzhou, China.
Angewandte Chemie (International ed. in English)
|October 18, 2023
概括
研究人员在Panus rudis中确定了PanhH酶,该酶负责制造环氧 (ECH) 化合物. 这一发现揭示了菌中ECH生物合成的新型,独立的进化途径.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 自然产品生物合成 自然产品生物合成
背景情况:
- 环氧环色 (ECH) 部分存在于来自细菌的天然产品中,这些产品包括ascomycetes和basidiomycetes.
- 在细菌和ascomycetes中已知用于ECH形成的酶,但在basidiomycetes中的生物合成途径仍然没有表征.
研究的目的:
- 为了确定基因基因基因基因的panepoxydone生物合成的基因基因基因基因基因基因基因基因基因基因基因 Panus rudis.
- 在生物化学上描述参与ECH形成的细胞染色体P450酶,PanH.
- 探索基质特异性和基本菌体中ECH生物合成的进化起源.
主要方法:
- 在Panus rudis.中的遗传部位识别.
- 使用酵母生物催化剂对细胞染色体P450酶PanH的生物化学表征.
- 使用产生泛H的酵母合成ECH化合物.
- 同性学建模,分子动力学模拟和局部导向突变发生.
主要成果:
- 在Panus rudis中发现了一种负责panepoxydone生物合成的基因位点.
- 细胞染色体P450酶PanH在生物化学上被描述为环氧化物形成的催化剂.
- 使用PanH作为生物催化剂合成了一个生物活性ECH化合物的库.
- 结构分析揭示了PanH的基质特异性及其与已知的ECH形成酶的进化分歧.
结论:
- 这项研究阐明了之前未知的ECH生物合成途径.
- PanH代表了一种用于ECH形成的新型酶类,这表明了融合进化.
- 这项工作为生物催化生产各种ECH化合物开辟了道路.
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