新型菌通过TOR介导的脂不对称性重塑来适应宿主环境
Laura C Ristow1, Andrew J Jezewski1, Benjamin J Chadwick2
1Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Nature communications
|October 18, 2023
概括
加密球菌新型人适应宿主CO2涉及TOR途径和Ypk1,影响膜不对称. 一种载体,PDR9,影响CO2的敏感性和适应性,显示出与S. cerevisiae不同的脂质调节.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 病原体适应 病原体适应
背景情况:
- 这是Cryptococcus spp. 脑膜炎是环境真菌引起脑膜炎在免疫受损的个体.
- 主体二氧化碳度明显高于外部环境,对真菌病原性至关重要.
- 适应宿主条件,特别是二氧化碳水平,是引起疾病的先决条件.
研究的目的:
- 研究Cryptococcus neoformans适应主机二氧化碳度的基础分子机制.
- 为了确定关键的途径和基因参与二氧化碳耐受性和病原性.
- 为了比较C. neoformans中的脂质不对称调节与Saccharomyces cerevisiae中的脂质不对称调节.
主要方法:
- 基因查以确定参与CO2适应的基因.
- 转录造型分析在不同的CO2水平下分析基因表达变化.
- 血脂质不对称性和输送器功能的生物化学分析.
主要成果:
- 通过Ypk1的TOR途径对于C. neoformans通过重塑酸胺异对称性来适应宿主CO2至关重要.
- 一种C. neoformans的ABC/PDR转运体,PDR9,在对CO2敏感的菌株中被上调,抑制了CO2诱导的脂质重塑,并增加了CO2的敏感性.
- 通过TOR-Ypk1轴调节血脂质不对称的调节在C. neoformans和S. cerevisiae之间有所不同.
- 主体二氧化碳水平抑制了对温度 (Mpk1) 和pH (Rim101) 响应的C. neoformans通路.
结论:
- TOR-Ypk1通路对于C. neoformans通过调节血脂质不对称来适应宿主CO2至关重要.
- PDR9在二氧化碳耐受性和适应性中发挥着重要作用,突出显示了传送器功能的重要性.
- 为了使C. neoformans成功适应宿主,需要在对不同宿主压力的反应之间保持微妙的平衡,包括CO2,温度和pH.
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