父亲DNA甲基化在米细胞中被重塑为母体水平
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Nature communications
|October 18, 2023
概括
在大米杂交体中,父DNA甲基化被重置为与母细胞中的母细胞水平相匹配,支持母细胞基因表达. 在早期胚胎发育过程中,以代基因为特征的甲基化模式后来被重新建立起来.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发育生物学是发展生物学.
背景情况:
- 表观遗传重编程对于在生殖过程中重置基因组至关重要,以便早期发育.
- 了解植物胞体中的亲基甲基化动态对于理解遗传和发育至关重要.
- 在开花植物中DNA甲基化重塑的机械细节在很大程度上是未知的.
研究的目的:
- 调查大米杂交胚胎和早期胚胎中的等位基因特异性DNA甲基化模式.
- 为了阐明受精后父亲DNA甲基化重编程.
- 了解在胚胎基因组激活 (ZGA) 过程中DNA甲基化和基因表达之间的关系.
主要方法:
- 在大米杂交胚胎和发育中的胚胎中对异位基因特异性DNA甲基化的分析.
- 父母等位基因之间的甲基化模式的比较.
- 甲基化模式与基因素修饰的相关性.
主要成果:
- 父亲DNA甲基化主要是重建,以匹配米细胞中的母性基水平.
- 这种母性偏差的甲基化模式在第一个胚胎分裂之后仍然存在.
- 在球状胚胎阶段,基因基因特异性甲基化和基因素修饰模式被重新建立.
结论:
- 父亲的DNA甲基化被重塑,使其与母亲的模式保持一致,而这是在米中生殖细胞基因组重编程过程中发生的.
- 染色体记忆机制可能会在杂交物中保持亲代基因特异性甲基化.
- 这些发现提供了关于表观遗传和植物早期发育的见解.
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