核蛋白下调调节调节原生体分化,独立于核孔数
Amy E Neely1, Yang Zhang2,3, Laura A Blumensaadt1
1Department of Molecular Biosciences, Northwestern University, Evanston, IL, 60208, USA.
Communications biology
|October 18, 2023
概括
核素93 (NUP93) 在角质细胞的下调影响分化和屏障功能. 这项研究表明,NUP93敲击影响基因表达和免疫反应,独立于核孔数.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
背景情况:
- 核素 (NUPs) 形成核孔复合体,对核细胞质运输至关重要.
- 在人类角质细胞分化过程中,大多数NUP,特别是NUP93的NUP显著下调.
- 在角质细胞分化过程中NUP下调的功能后果尚未完全理解.
研究的目的:
- 调查NUP93下调在角质细胞分化中的作用.
- 为了确定降低NUP水平是否会影响核孔数和透性.
- 阐明NUP93影响状细胞基因表达和功能的分子机制.
主要方法:
- 随机光学重建显微镜 (STORM) 用于量化核孔数.
- 在初级人类角质细胞中NUP93的敲击.
- 转录组分析 (RNA测序) 用于分析基因表达变化.
- 通过NF-κB记者测定和免疫光检测来评估转录因子活性.
主要成果:
- 尽管NUP下调,但核孔数没有显著变化.
- NUP93敲击损害了角质细胞的克隆原性和表皮再生能力.
- NUP93敲除诱导分化基因,激活NF-κB信号通路.
- 观察到NF-κB p65/p50转录因子的核局部增加.
结论:
- 不同的NUP表达水平在角质细胞分化中起着基因调节作用.
- NUP93在角质细胞分化中的功能与其在核孔数调节中的作用是独立的.
- 通过NF-κB通路的激活,NUP93 knockdown会影响状细胞屏障功能.
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