甘酸C2对循环胺诱导的免疫抑制产生药理作用:一项涉及分子对接和实验验证的研究
Yuchen Liu1, Dongsheng Tan2, Hong Cui1
1School of Life Science and Technology, Harbin Normal University (Songbei Campus), No. 1, Shida Road, Hulan District, Harbin, 150025, Heilongjiang Province, China.
Scientific reports
|October 18, 2023
概括
来自Ganoderma lucidum的甘酸C2 (GAC) 通过调节免疫路径,有效地对抗循环胺 (CY) 诱导的免疫抑制. 这项研究确定了GAC.
科学领域:
- 药理学和自然产品的研究.
- 免疫学和免疫药理学
- 生物信息学和计算生物学
背景情况:
- 光 (Ganoderma lucidum) 的发酵提取物含有具有免疫调节功能的三烯酸.
- 环胺 (Cyclophosphamide,CY) 是一种已知诱导免疫抑制的化疗剂.
- 甘酸C2 (GAC) 是一种具有潜在治疗应用的关键三类化合物.
研究的目的:
- 调查甘酸C2 (GAC) 对循环胺 (CY) 诱导的免疫抑制的药理作用.
- 通过生物信息学,分子对接和实验验证的结合,阐明GAC的潜在治疗机制.
主要方法:
- 从公共数据库 (DisGeNET,CTD,GeneCards,PharmMapper) 中识别与GAC相关的目标基因.
- 使用STRING数据库构建蛋白质-蛋白质相互作用网络.
- 分子对接以预测GAC-蛋白相互作用.
- 在CY诱导免疫抑制模型中使用ELISA和qRT-PCR进行实验验证.
主要成果:
- 确定了56个与CY诱导的免疫抑制相关的与GAC相关的标.
- 丰富性分析表明,它参与了免疫和炎症反应途径.
- 确定了STAT3和TNF作为核心目标,其中GAC显示出有利的结合亲和力.
- 动物实验证实了GAC在免疫抑制模型中提高免疫力和STAT3 / TNF基因表达的能力.
结论:
- 甘酸C2证明了对CY诱导的免疫抑制的治疗潜力.
- 这些机制涉及STAT3和TNF信号通路的调节.
- 这些发现为开发用于增强免疫力的Ganoderma lucidum衍生产品提供了理论基础.
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