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在Bacillus subtilis中优化了甲基供体和减少了前体降解途径,用于在Bacillus subtilis中生产基甲基基氨

Xian Yin^1,2,3, Meiyi Zhao^1,3, Yu Zhou^1,3

¹Key Laboratory of Geriatric Nutrition and Health (Ministry of Education), Beijing Technology and Business University, Fucheng RD 11, Beijing, 100048, China.

Microbial cell factories

|October 18, 2023

概括

这项研究通过优化甲基的供应和抑制前体降解,增强了Bacillus subtilis中的-甲基氨基 (SeMCys) 产量. 这导致了迄今为止获得的最高的SeMCys产量,为代谢提供了洞察力.

科学领域:

微生物学微生物学
生物化学生物化学
代谢工程是代谢工程.

背景情况:

单甲基单半氨酸 (SeMCys) 具有抗癌性质,可以减轻神经系统缺陷.
之前的工作是通过操纵S-adenosylmethionine合成和氨基胺甲基转移酶表达来为SeMCys生产工程Bacillus subtilis (GBACB).
这项研究旨在通过增加甲基氨酸的可用性和减少前体降解来进一步提高SeMCys的生产.

研究的目的:

为了改善SeMCys在工程 Bacillus subtilis中的生产.
为了研究甲基氨酸作为甲基供体的作用.
识别和抑制参与前体降解的途径.

主要方法:

评估了三种氨酸S-甲基转移酶用于氨酸生产.
通过整合NmMmt基因来改造细菌细菌.
鉴定和淘汰的基因 (iscSB,sdaA) 参与了单半氨酸和氨酸降解途径.
优化培养条件,包括化的度.

主要成果:

整合NmMmt使SeMCys的产量从20.7增加到687.4μg/L.
淘汰scSB使SEMCys的产量翻了一番.
删除sdaA提高了SeMCys的产量,达到4120.3μg/L.
在优化条件下,产量为7.5 mg/L SeMCys.

结论:

甲基氨酸对于SeMCys在Bacillus subtilis中的产生至关重要.
增加前体供应可显著提升SeMCys的合成.
这项研究实现了创纪录的SeMCys产量和对代谢的先进理解.

关键词:

这种细菌是 Bacillus subtilis.甲基氨酸是什么甲基氨酸是什么单甲基单半氨酸是什么单甲基单氨酸是什么单半氨酸是一种单半氨酸.血清蛋白 (Serine) 是一种蛋白质.

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