在Cenchrus ciliaris L L的细胞悬浮中通过体质胚胎生成进行植物再生
Shipra Goyal1, Vijaya Chatterjee2, Vishvas M Kulkarni3
1Plant Developmental Biology, Department of Botany, University of Delhi, Delhi, 110007, India.
Plant methods
|October 18, 2023
概括
这项研究建立了用于Cenchrus ciliaris L.再生的细胞悬浮培养,克服了改善植物繁殖和遗传转换潜力的传统方法的局限性.
科学领域:
- 植物生物技术 植物生物技术
- 细胞生物学 细胞生物学
- 农业科学 农业科学
背景情况:
- 细胞悬浮培养提供均的细胞系,使营养素获得均.
- 它们是适合进行基因转换的有能力细胞的理想来源.
- 这项研究的重点是通过细胞悬浮的Cenchrus ciliaris L.再生.
研究的目的:
- 在Cenchrus ciliaris L.中建立和维护细胞悬浮培养.
- 诱导体质胚生成用于体外植物再生.
- 克服质介导再生的局限性.
主要方法:
- 在MS媒介上从未成熟的花朵开始发芽的,含有2,4-D和BAP.
- 建立和维护细胞悬浮培养与定义的亚培养间隔.
- 使用特定的介质添加剂诱导体质胚生成和芽再生.
- 使用RAPD原料进行幼苗发育,适应和遗传忠实性分析.
主要成果:
- 细胞悬浮培养物建立并保持20天的亚培养间隔和11天的细胞翻倍时间.
- 实体胚胎生成和芽生长再生在MS介质上实现了最高的再生,补充了2,4-D,BAP,素水解剂,谷氨酸和プロ林.
- 再生植物表现出对母植物的遗传忠诚,适应生存率为60%.
结论:
- 细胞悬浮培养促进了C. ciliaris.在体外有效的植物再生.
- 身体胚胎发生的进展导致成功的发芽和根部发育.
- 这种方法克服了与衰老相关的再生潜力限制.
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