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精子中皮特异性转录不孕患者的基因甲基化状态:系统性审查和元分析
Rossella Cannarella1,2, Claudia Leanza1, Andrea Crafa1
1Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
The world journal of men's health
|October 19, 2023
概括
在不孕男性中,MEST基因的精子DNA甲基化显著更高. 这一发现表明,MEST基因甲基化可能是辅助生殖技术 (ART) 的表观遗传风险因素.
科学领域:
- 表观遗传学和生殖生物学
- 精子动物DNA甲基化分析分析
- 在胚胎发育中的印记基因功能
背景情况:
- 介质体特异性转录 (MEST) 基因是对胚胎生存至关重要的父性表达的印记基因.
- 之前的元分析表明,与肥沃对照人群相比,不孕男性的精子中MEST基因甲基化升高.
- 了解MEST甲基化模式对于评估男性不孕症和生殖结果至关重要.
研究的目的:
- 对精子中MEST基因甲基化模式进行更新的系统审查和元分析.
- 为了比较具有异常精子参数的男性和具有正常参数的男性之间的MEST甲基化水平.
- 评估MEST基因甲基化与男性不孕症之间的关联.
主要方法:
- 在PROSPERO注册的系统审查和元分析 (CRD42023397056).
- 包括评估MEST基因甲基化在不孕或有异常精子参数的男性与对照者的精子中的原始研究.
- 来自6项涉及301名患者和163名对照组的研究数据的定量综合.
主要成果:
- 与对照组相比,在不育患者中观察到显著更高的MEST基因甲基化水平 (SMD 2.150,p=0.017).
- 研究之间发现了很高的异质性 (I2=97.91%),这表明研究结果的变化.
- 转基因回归显示MEST甲基化水平独立于患者年龄和精子度.
结论:
- 精子DNA甲基化,特别是MEST基因,可能是辅助生殖技术 (ART) 的表观遗传风险因素.
- 对于接受ART的夫妇来说,MEST基因值得考虑将其纳入遗传小组.
- 进一步的研究可以确定成本有效的基因来分析ART中的表观遗传因素.
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