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对Raf1和Bsd2在六边形Rubisco组件中的功能进行结构洞察

Ran Wang¹, Hui Song¹, Wenjuan Zhang¹

¹State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100101, China.

Molecular plant

|October 19, 2023

概括

研究人员重建了Synechococcus sp. 的成分. PCC6301 在实验室中使用沙佩罗宁系统的鲁比斯科. 阿拉比多普西斯塔利亚纳 (At) 组装因子AtRaf1和AtBsd2有助于鲁比斯科全酶的生产和稳定,提出了新的生物发生路径.

科学领域:

生物化学和分子生物学
酶学是一种酶学.
结构生物学结构生物学

背景情况:

利布洛斯-1,5-双酸碳氧化酶/氧化酶 (Rubisco) 是全球最丰富的酶,对碳固定至关重要.
为提高催化效率而设计Rubisco是提高农业生产力的关键目标.
在体外和外来宿主中,六角体鲁比斯科的折叠和组装方面的挑战阻碍了基因操纵.

研究的目的:

为了使Synechococcus sp.的复制. PCC6301 鲁比斯科全酶在体外.
为了研究蓝菌和Arabidopsis thaliana (At) 组装因子在鲁比斯科生物生成中的作用.
阐明鲁比斯科组件的结构基础,并提出生物发生的途径.

主要方法:

在实验室中,Rubisco全酶的复制使用蓝藻细菌的Chaperonin系统.
包括阿拉比多opsis thaliana (At) 组装因子:鲁比斯科积累因子1 (AtRaf1) 和捆盖缺陷-2 (AtBsd2).
电子显微镜 (Cryo-EM) 用于确定Rubisco-chaperone复合物的结构.

主要成果:

鲁比斯科全酶在体外成功地用蓝藻细菌Raf1产生,并进一步使用AtRaf1和AtBsd2.
与原生生物系统相比,冷-EM结构显示RbcL和AtRaf1之间的相互作用较宽松.
发现AtBsd2可以稳定RbcL的灵活区域,包括终点和特定循环.

结论:

阿拉比多普西斯塔利亚纳的组合因子可以促进在体外复制蓝菌Rubisco.
在RbcL-Raf1相互作用中的结构差异表明原生生物和真核生物系统之间存在不同的组装机制.
这项研究基于结构和功能数据,为原生生物和真核生物鲁比斯科提出了新的生物发生途径.

关键词:

鲁比斯科 (Rubisco) 是一个古老的葡萄酒.组装因子的组装因子陪伴者是一个陪伴者.蛋白质组合组件的组合蛋白质折叠蛋白质的折叠

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