颗粒细胞殖民地刺激因子对全基外围血液干细胞移植捐赠者的基因毒性的影响:一项前性病例控制研究
Hasan Fatih Çakmaklı1, Hafize Gökçe1, Esma Söylemez2
1Department of Pediatric Hematology, Ankara University Faculty of Medicine, Ankara.
The Turkish journal of pediatrics
|October 19, 2023
概括
颗粒细胞殖民地刺激因子 (G-CSF) 在外围血液干细胞捐赠者中显示出基因毒性作用,对核分裂指数产生短期影响,对Olive的尾部动量产生长期影响. 需要进一步的研究来证实这些发现.
科学领域:
- 血液学 血液学 血液学
- 基因毒理学 基因毒理学
- 干细胞移植 干细胞移植
背景情况:
- 每年有成千上万的捐赠者接受G-CSF用于干细胞动员.
- 之前关于G-CSF基因毒性的研究得出了不确定的结果.
- 这项研究使用多种方法前性评估了PBSC捐赠者的G-CSF基因毒性.
研究的目的:
- 评估G-CSF在外周血液干细胞 (PBSC) 供体中的基因毒性作用.
- 调查短期和长期的基因毒性影响.
- 为正在进行的关于G-CSF安全的辩论提供新数据.
主要方法:
- 对36名接受G-CSF的PBSC捐赠者的前性评估.
- 使用微核试验 (MNT),核分裂指数 (NDI) 和彗星试验 (CA) 进行基因毒性评估.
- 在三个时间点收集的血液样本:G-CSF前,G-CSF后和G-CSF后一个月.
主要成果:
- 橄的尾部时刻 (OTM) 在G-CSF后一个月显著增加.
- 核分裂指数 (NDI) 在G-CSF后显著下降,但后来恢复到基线以上.
- 微核测试 (MNT) 和大多数彗星测试参数没有随着时间的推移显著变化.
结论:
- 在健康的捐赠者中,G-CSF表现出基因毒性作用,影响NDI短期和OTM长期.
- 这些发现为G-CSF基因毒性提供了新的见解.
- 支持需要进行更大规模的研究,并延长随访时间,以确认这些基因毒性影响.
关键词:
彗星测试试验 彗星测试试验基因毒性测试是基因毒性测试.颗粒细胞殖民地刺激因子血液造血干细胞供体细胞的供体.造血干细胞动员 造血干细胞动员微核试验是微核试验.周围血液干细胞移植组织捐赠者的组织捐赠者更多相关视频
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