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升级调节的microRNA-429通过向HIF1AN和调节HIF1A/VEGF通路来赋予子宫内膜层细胞功能障碍

Rong Zheng¹, Yulan Liu¹, Yan Lei¹

¹Department of Gynecology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, Hubei, China.

Open medicine (Warsaw, Poland)

|October 19, 2023

概括

抑制microRNA-429 (miR-429) 通过向低氧诱导因子1亚单元α抑制剂 (HIF1AN) 并调节HIF1A/VEGF通路,阻碍细胞入侵和病变发展,从而抑制子宫内膜异位症的进展.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
疾病的细胞机制.

背景情况:

子宫内膜异位症 (EM) 是一种常见的雌激素依赖性疾病,影响女性的生活质量.
微RNA-429 (miR-429) 在EM病原体中的作用已被建议,但尚未完全理解.
澄清miR-429的分子机制对于理解EM至关重要.

研究的目的:

为了研究 miR-429 在子宫内膜异位症中的分子机制.
确定miR-429在人类子宫内膜层细胞 (HESC) 行为的作用.
评估miR-429调制对实验性子宫内膜异位症模型的影响.

主要方法:

通过免疫光学和流细胞计测定HESCs的识别.
通过子宫内膜自身移植建立了对子宫内膜症的小鼠模型.
基因和蛋白质表达的评估使用RT-qPCR和西式涂抹.
在体外功能测定 (扩散,迁移,入侵) 和光酶记者测定.
在体内研究涉及小鼠模型中的miR-429枯竭.

主要成果:

抑制miR-429在体外显著降低了HESC的扩散,迁移和侵入性.
miR-429直接针对低氧诱导因子1亚单元α抑制剂 (HIF1AN) 在HESC中.
HIF1AN沉默可以逆转miR-429抑制对HIF1A/VEGF信号通路的抑制作用.
在子宫内膜异位症的小鼠模型中,miR-429的耗尽减轻了子宫外病变的发展.

结论:

抑制miR-429阻碍了HESC侵入性行为和子宫内膜异位症的进展.
该机制涉及准HIF1AN并调节HIF1A/VEGF信号通路.
miR-429代表了对子宫内膜异位症的潜在治疗标.

关键词:

在HIF1A/VEGF路径中.这就是HIF1AN.胚胎内膜的 stromal 细胞.子宫内膜异位症是什么?子宫内膜异位症是什么在 miR-42929 中使用.

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