处理和储存方法会影响口腔和肠道微生物组合的组成
Dorothy K Superdock1, Wei Zhang2, Angela C Poole1
1Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States.
Frontiers in microbiology
|October 19, 2023
概括
微生物组样本的储存和处理显著影响结果. 化便和新鲜唾液样本显示出更高的多样性,影响了微生物组成和研究比较.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 微生物组研究依赖于准确的微生物社区概况.
- 不一致的样本处理可以在微生物组数据中引入偏差.
- 标准化协议对于可靠和可比的结果至关重要.
研究的目的:
- 评估不同储存和加工方法对微生物组合的影响.
- 评估样本处理如何影响微生物多样性和社区结构.
- 为优化微生物组样本分析提供指导.
主要方法:
- 收集了来自10个人的便,唾液和牙抽样.
- 评估了四种便存储/加工方法,并将唾液/牙样本与新鲜对照进行了比较.
- 利用16S rRNA基因测序来分析微生物社区的多样性和组成.
主要成果:
- 解热化便,新鲜的整体唾液和解的牙上游液显示出最高的α多样性.
- 化便样本显示了阿基亚菌的流行率增加和更高的Firmicutes/Bacteroidetes比率.
- 在各种储存和加工条件下观察到ASV丰度的差异.
结论:
- 储存和加工方法显著影响微生物组分析结果.
- 建议对便样本进行冷化,对口腔样本进行新鲜/超养分.
- 标准化样本处理对于避免微生物组研究中的混因素至关重要.
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