通过立方斜线PSF建模来提高ObSTORM成像性能

通过立方斜线PSF建模来提高ObSTORM成像性能

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Donghoon Koo¹, Minchol Lee¹, Youngseop Lee¹

¹Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea.

Biomedical optics express

|October 19, 2023

概括

一个新的立方线模型改进了斜平面超分辨率显微镜 (obSTORM) 对于厚厚的生物样本. 这种增强的点传播函数 (PSF) 模型增加了成像分辨率和轴距,使组织的3D成像速度更快.

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
超高分辨率的成像技术

背景情况:

斜平面显微镜 (obSTORM) 为厚厚的生物标本提供超高分辨率成像.
现有的高斯点分布函数 (PSF) 模型限制了 obSTORM 解析度和轴向定位范围,原因是 PSF 的形状不合适.
这限制了obSTORM在成像复杂的生物结构中的应用.

研究的目的:

为 obSTORM.开发一个更准确的点传播函数 (PSF) 模型.
为了提高3D定位精度和提高体积成像能力.
在 obSTORM 中克服高斯式 PSF 模型的局限性.

主要方法:

在 obSTORM.中实现立方支线,以精确建模实验性PSF形状.
精制的PSF模型应用于超分辨率成像小鼠视网膜组织.
对成像分辨率,定位精度和轴距改善的定量分析.

主要成果:

与高斯模型相比,立方线PSF模型显著提高了3D定位精度.
图像分辨率在老鼠视网膜组织中增加了大约1.2倍.
单个分子的无拼接和截面间隔的翻倍是通过体积阻塞实现的.

结论:

立方线PSF建模为准确和高效的OBSTORM成像提供了一种卓越的方法.
这一进步使得生物标本的分辨率更高,体积成像更快.
这种精细的模型扩展了obSTORM对研究厚组织和小动物的实用性.

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