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生物细胞和亚细胞结构的体积细分用于光学衍射断层扫描图像的图像

Martyna Mazur¹, Wojciech Krauze¹

¹Warsaw University of Technology, 8 Boboli Str., Warsaw, 02-525, Poland.

Biomedical optics express

|October 19, 2023

概括

一种新的半自动方法 - - 光衍射断层扫描细分分析软件 (ODT-SAS) - - 从3D成像数据中准确地细分细胞和器官. 这种技术在生物医学研究的现有方法上显示出优越性.

科学领域:

生物医学研究的研究.
定量成像技术定量成像
细胞生物学细胞生物学

背景情况:

光学衍射断层扫描 (ODT) 提供了细胞和内部结构的3D定量成像.
准确的3D细分ODT图像对于定量分析至关重要,但目前的方法具有挑战性.

研究的目的:

介绍ODT-SAS,一种新的半自动方法,用于在ODT图像中对细胞,细胞核和脂质结构 (LS) 进行细分.
评估ODT-SAS的性能与现有的细分技术相比.

主要方法:

ODT-SAS使用非机器学习技术的组合进行细分.
进行了对Cellpose进行细胞细分的比较分析,以及用于器官细分的手动切片逐切片细分.

主要成果:

与Cellpose相比,ODT-SAS在细胞细分方面表现优越.
该方法显示了细胞,细胞核和脂质结构的准确检测的巨大潜力.

结论:

在ODT成像中,ODT-SAS为准确的3D细分提供了有效的解决方案.
这种技术增强了生物医学研究中的定量分析能力,包括细胞和器官成像.

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