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在孕牛白细胞中,IFNT通过I型IFN信号通路刺激 lysosomal功能

Md Abdus Shabur Talukder^1,2, Ahmed Zaky Balboula³, Hanako Bai²

¹College of Agricultural Sciences (CAS), IUBAT-International University of Business Agriculture and Technology, Uttara, Dhaka, Bangladesh.

Reproduction (Cambridge, England)

|October 19, 2023

概括

干扰因子 (IFNT) 通过Janus激活激酶 (JAK) 途径激活牛白细胞中的溶解体. 这种IFNT介导的溶酶激活可能为牛早期怀孕检测提供了一种新方法.

科学领域:

生殖免疫学生殖免疫学
怀孕期间的细胞信号传递

背景情况:

干扰素 (IFNT) 对于在反动物中确定怀孕至关重要.
在子宫组织和外围血液白细胞 (PBL) 中,IFNT诱导干扰素刺激基因 (ISG).
之前的研究指出,孕牛的PBL中溶酶体和甲素 (CTS) 活性增加,但机制尚不清楚.

研究的目的:

调查乳牛PBL在怀孕早期的IFNT介导 lysosomal激活的机制.
探索Janus激活激酶 (JAK) 途径在PBL中的IFNT信号传递中的作用.

主要方法:

在人工授精 (AI) 后的第18天从霍尔斯坦牛中收集了PBL.
基因表达 (LAMP1,LAMP2,CTSB,CTSK) 和溶酶体活性在来自孕妇和非孕妇母牛的PBL中进行了分析.
用IFNT和JAK抑制剂治疗进行了PBL的体外刺激.

主要成果:

在孕母牛的PBL中,LAMP1,LAMP2,CTSB和CTSK的基因表达显著更高.
在体外IFNT刺激增加了溶酶体酸化和LAMP1/2的表达,以及CTSB/CTSK活性.
抑制JAK可以减少IFNT诱导的溶酶体变化和基因表达.

结论:

IFNT通过涉及JAK信号的1型干扰素介导途径刺激PBL中的 lysosomal激活.
在PBL中IFNT介导的溶酶体激活可能是牛早期怀孕检测的新生物标志物.

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