血细胞外膀LncRNA H19作为潜在的炎症性肠道疾病的诊断生物标志物
Raheleh Heydari1, Sara Fayazzadeh2, Shabnam Shahrokh3
1Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Inflammatory bowel diseases
|October 19, 2023
概括
这项研究确定了循环细胞外囊泡长非编码RNA H19 作为诊断活性炎症性肠病 (IBD) 的有希望的非侵入性生物标志物. 这些发现表明H19可以有效地区分活跃IBD与缓解,健康个体和其他胃肠道疾病.
科学领域:
- 胃肠道学和分子诊断学
- 生物标志物发现发现
- 细胞外囊泡研究研究
背景情况:
- 炎症性肠病 (IBD),包括克罗恩氏病和性结肠炎,通过各种临床和侵入性方法进行诊断.
- 细胞外囊泡 (EVs) 促进细胞间通信,并且越来越多地被认为是它们在疾病中的作用.
- 由于其稳定性和疾病特异性表达,EV中的长非编码RNA (lncRNA) 显示出作为诊断生物标记物的潜力.
研究的目的:
- 研究5种特定的lncRNAs在IBD患者和健康对照的组织和血EV中的表达.
- 评估这些EV-lncRNAs作为活性IBD的非侵入性诊断生物标志物的潜力.
- 确定一种特定的EV-lncRNA,能够区分活跃IBD和缓解期IBD,类风湿性关节炎和刺激性肠综合征.
主要方法:
- 使用定量实时聚合酶连锁反应 (qRT-PCR) 来测量组织和血EV中的lncRNA表达.
- 机器学习和接收器操作特征 (ROC) 曲线分析被用来评估诊断准确性.
- 使用动态光散射,显微镜和西部斑点,证实了EV隔离.
主要成果:
- 在活性IBD病变中,CDKN2B-AS1,GAS5和TUG1 lncRNAs的下调,而H19和CRNDE的上调.
- 在血EVs中成功检测到H19表达.
- H19在区分活跃IBD和缓解期IBD (AUC=0.95),健康对照 (AUC=0.97),类风湿关节炎 (AUC=1.00) 和刺激性肠综合征 (AUC=0.97) 中表现出高准确度.
结论:
- 循环的细胞外囊泡衍生 lncRNA H19 显示出作为一个非侵入性生物标志物具有重要的潜力,用于积极的IBD诊断.
- 这些发现支持EV-lncRNAs在区分IBD与其他胃肠道和自身免疫疾病中的实用性.
- 需要进一步验证以确定EV-lncRNA H19在IBD的临床诊断实践中.
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