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用内源性斯特雷普塔维丁/FLAG标签在小鼠体内内运输 (IFT) 蛋白的亲和性净化

Tina Beyer1, Tiago Martins2, Jeshmi Jeyabalan Srikaran2

  • 1Institute for Ophthalmic Research, Center for Ophthalmology, University of Tübingen, Tübingen, Germany.

Methods in molecular biology (Clifton, N.J.)
|October 19, 2023
PubMed
概括

研究人员开发了一种使用基因编辑创建标记小鼠模型的新方法,用于研究特定组织中的蛋白质-蛋白质相互作用. 这种方法可以在生理条件下更深入地了解生物复杂性.

关键词:
关联性净化净化方法在C57Bl6/N小鼠中.这就是Cas9的情况.基因编辑是一种基因编辑.质谱测量质量谱测量蛋白质复合体是一种蛋白质复合体.斯特雷普塔维丁/FLAG标签在gRNARNA中.

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