NAC转录因子SlNOR-like1在番茄果皮膜的形成中起着双重的调节作用
Gang-Shuai Liu1, Hua Huang2, Donald Grierson3,4
1Laboratory of Fruit Biology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Journal of experimental botany
|October 19, 2023
概括
该NAC转录因子SlNOR-like1调节番茄果皮的发育. 它促进皮质沉积,同时抑制积累,提供了皮质形成的新模式.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 植物皮质是一个重要的保护屏障,由皮质和组成.
- 我们对水果皮质形成的转录调节的理解是有限的.
研究的目的:
- 研究NAC转录因子SlNOR-like1在番茄果皮膜发育中的作用.
- 阐明控制皮膜形成的转录调节网络.
主要方法:
- 通过CRISPR/Cas9基因编辑,在番茄中产生类似SlNOR的1个淘汰突变体.
- 对皮质沉积,皮质厚度和积累的分析.
- 促进体结合试验和基因表达分析以确定类似SlnOR的1标.
主要成果:
- 类似SlNOR的1淘汰突变体显示了减少的皮质沉积和皮质厚度,具有微裂纹.
- 在SlNOR样1突变体中促进了积.
- 类似于SlNOR的1直接激活生物合成基因 (SlGPAT6,SlCD2) 并抑制生物合成/运输基因 (SlKCS1,SlCER1-2,SlWAX2,SlLTPG1-like).
结论:
- SlNOR-like1在番茄果皮皮的发育中起着双重作用,促进皮皮和抑制.
- 这项研究提出了一种用于转录调节水果皮膜形成的新型模型.
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