异常和剂量依赖的表皮病导致番茄果实大小的变化
Lyndsey Aguirre1, Anat Hendelman2, Samuel F Hutton3
1Cold Spring Harbor Laboratory, School of Biological Sciences, Cold Spring Harbor, NY, USA.
概括
使用番茄植物的调节性DNA研究了基因之间的相互作用. 这些调节元件的遗传变异揭示了影响水果大小的复杂,不可预测的相互作用.
科学领域:
- 遗传学
- 植物生物学
- 进化生物学
背景情况:
- 传统的表皮病研究侧重于影响蛋白质活性的突变.
- 大多数自然遗传变异发生在 cis 调节性 DNA 中,对基因表达和功能产生定量影响.
- 对于解释表型变异至关重要的是了解 cis 调节元素中的表位.
研究的目的:
- 系统地评估控制番茄果实大小的表观关系,使用自然和人工调节基.
- 量化 cis 调节性等位基因强度如何改变表皮质.
- 探索基因调节网络中的 cis 调节多样性的作用.
主要方法:
- 在植物干细胞电路中使用促进器等位列和两个额外的位点.
- 收集了来自46个基因型的3万多个表型数据点.
- 分析了剂量依赖关系和异位基因特异性相互作用.
主要成果:
- 揭示了和调节基因和果实大小之间的剂量依赖关系.
- 确定了基因特异性的特异性相互作用,包括与化驱动的果实大小变化相关的相互作用.
- 已证明非线性和不可预测的相互作用来自于cis调节性等位基因多样性.
结论:
- 细胞调节变异代表了未被充分研究的表皮病源.
- 基因调节网络中的等位基因多样性可以导致复杂的非线性表型结果.
- 这项研究为研究自然遗传变异中的表观症提供了一个新的框架.
相关概念视频
Epistasis
46.9K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
46.9K
Epistasis Analysis
5.0K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.0K
Dihybrid Crosses
75.0K
Overview
75.0K
Law of Independent Assortment
55.8K
While Mendel’s Law of Segregation states that the two alleles for one gene are separated into different gametes, a different question of how different genes are inherited remains. For example, is the gene for tall plants inherited with the gene for green peas? Mendel asked this question by experimenting with a dihybrid cross; a cross in which both parents are homozygous for two distinct traits resulting in an F1 generation that are heterozygous for both traits.
55.8K
Trihybrid Crosses
23.4K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.4K
Monohybrid Crosses
230.2K
Overview
230.2K


