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在脂多糖刺激下,在的巨中对circSTX8的功能分析

Jishuang Tan¹, Yue Lu¹, Huan Li²

¹College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Research in veterinary science

|October 19, 2023

概括

循环RNA STX8 (circSTX8) 在用脂多糖 (LPS) 刺激的巨中加剧细胞损伤和炎症. 这种循环RNA在调节免疫反应中起作用.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 是基因表达,发育,分化和免疫反应的关键调节者.
在之前的研究中,循环RNA STX8 (circSTX8) 在脂多糖 (LPS) 刺激期间在的肺部表达较低.
circSTX8是由STX8基因的56前子的逆拼接生成的,是稳定的分子.

研究的目的:

研究巨中circSTX8的作用和功能.
为了确定circSTX8对脂聚糖 (LPS) 诱导的细胞损伤和炎症的影响.
确定由circSTX8.8调节的潜在分子机制和途径.

主要方法:

使用RT-qPCR和桑格测序来识别和量化circSTX8.8.
RNase R外核酶治疗证实了circSTX8.8的稳定性.
的巨被操纵为circSTX8过度表达,并用LPS治疗.
细胞计数工具-8 (CCK8) 和RT-qPCR评估了细胞活力和促炎性细胞因子表达.

主要成果:

circSTX8在各种组织中高度表达,包括盲体,脏和肺部,但不在肌肉或大脑中.
过度表达circSTX8显著加剧了LPS诱导的细胞损伤,减少了细胞活力.
circSTX8在LPS刺激的巨中增加了促炎细胞因子的表达.
确定了四种与circSTX8相互作用的miRNA,它们准了914个涉及紧结,mTOR,MAPK和TGF-β信号传递等途径的基因.

结论:

circSTX8在加剧巨中LPS诱导的细胞免疫和炎症反应方面发挥着重要作用.
circSTX8通过与特定的miRNA和下游信号通路的相互作用来调节这些反应.
这些发现提供了关于circRNAs在禽类免疫和炎症中的作用的见解.

关键词:

在HD11细胞中,免疫反应的免疫反应.炎症炎症是一种炎症.脂聚糖类糖化物脂聚糖类糖化物 circSTX8 在线观看这就是miRNAs.

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