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在卵细胞成熟期间低剂量脂聚糖暴露会破坏早期的牛胚胎发育

B Castro¹, J I Candelaria², M M Austin¹

¹Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM, 88003, USA.

|October 19, 2023

概括

低剂量的脂聚糖 (LPS) 不影响牛卵细胞成熟或早期胚胎发育. 然而,LPS暴露降低了卵细胞对芽细胞发育的能力,这表明对生育能力的潜在影响.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
动物科学动物科学

背景情况:

来自格拉姆阴性细菌的脂聚糖 (LPS) 可以破坏卵巢功能并导致不孕.
亚临床LPS暴露对卵细胞能力和胚胎发育的影响需要进一步研究.

研究的目的:

研究低剂量脂聚糖 (LPS) 暴露对牛卵细胞和胚胎能力的影响.

主要方法:

牛卵细胞在体外成熟,使用不同剂量的LPS (0.01,0.1,1μg/mL) 或载体控制.
评估了卵细胞的核成熟度.
在体外受精后,使用传统显微镜和时隔成像来评估胚胎发育,分裂率,胚胎细胞率和细胞计数.

主要成果:

在治疗组之间没有观察到卵细胞核成熟 (GV,GVBD,MI,MII) 的显著差异.
通过显微镜评估的裂变率和胚芽细胞率没有显著差异.
虽然总胚胎细胞数量没有受到影响,但与对照组相比,LPS暴露 (特别是0.1μg/mL) 倾向于减少毛囊发育,并显著降低了胚胎细胞发育率.

结论:

低剂量的LPS暴露不会影响牛卵细胞的核成熟或初始胚胎分裂.
然而,经过体外受精后,LPS成熟的卵细胞对胚胎细胞发育的能力降低.
这些发现表明,牛中亚临床LPS暴露的潜在生育影响.

关键词:

胚胎囊中的胚胎细胞.疾病疾病疾病胚胎胚胎是一个胚胎.在体外成熟成长.脂聚糖类糖化物脂聚糖类糖化物卵细胞是卵细胞中的一个.

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