酸介质促进粉的体外消化:对宏分子拥挤效应的洞察
Yu-Long Sun1, Yi-Fan Tang2, Lei Wang2
1Molecular Food Science Laboratory, College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, China; ACON Biotech (Hangzhou) Co., Ltd., Hangzhou, China.
Food chemistry
|October 19, 2023
概括
酸 (NaCas) 通过稳定猪胰腺α-氨基酶 (PPA) 来提高粉的消化能力. 这种蛋白质拥挤效应改善了酶解水解和粉消化,突出显示了它在食品宏分子功能中的重要性.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 大分子相互作用的相互作用
背景情况:
- 多糖的宏分子拥挤会影响消化酶.
- 蛋白质拥挤对粉消化的影响尚不清楚.
研究的目的:
- 为了研究酸盐 (NaCas),粉和猪胰腺α-氨基酶 (PPA) 之间的相互作用.
- 确定NaCas对酶活性和粉消化能力的影响.
主要方法:
- 研究了NaCas与粉和PPA的相互作用.
- 评估了粉凝化,粉浸出和酶性水解率的变化.
- 使用结构比率分析了PPA的形状变化.
主要成果:
- NaCas延迟了粉的凝化,并减少了粉素的漏.
- 增加了易于消化的粉的比例.
- 促进了PPA的结构稳定性 (增加了有序结构),加速了酶性水解.
结论:
- NaCas通过宏分子拥挤效应提高了粉的消化能力.
- NaCas稳定了PPA,导致粉的加快水解.
- 大分子拥挤是食品大分子功能的重要因素.
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