探索Brevibacterium中的生物合成基因集群:对多样性和分布的比较基因组分析
Andrés Cumsille1, Néstor Serna-Cardona1, Valentina González1
1Centro de Biotecnología DAL, Universidad Técnica Federico Santa María, Valparaíso, Chile.
BMC genomics
|October 19, 2023
概括
探索各种Brevibacterium菌株,包括海洋隔离物H-BE7,揭示了新的物种和各种生物合成基因集群. 菌株H-BE7表现出抗菌活性,这表明发现天然产品的独特途径.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 自然产品的发现自然产品的发现
背景情况:
- 布雷维细菌 (Brevibacterium) 属是一种新型天然产品的来源.
- 探索各种生态系统对于发现微生物多样性和潜在的抗生素生产商至关重要.
- 从海洋沉积物中分离出来的H-BE7菌株被选择用于基因组分析,以评估其生物合成能力.
研究的目的:
- 对包括H-BE7在内的98种Brevibacterium菌株进行基因组分析,以了解它们的生物合成潜力.
- 为了在Brevibacterium属中识别新的物种.
- 调查生物合成基因集群 (BGCs) 在不同类别的分布,并确定类别特定的BGCs.
主要方法:
- 98个Brevibacterium菌株的全基因组测序和比较基因组分析.
- 基因组学分析用于构建基因组图和划分基因组.
- 平均核酸标识 (ANIb) 计算以确定潜在的新物种.
- 泛基因组分析用于研究核心和辅助基因.
- 对生物合成基因集群 (BGCs) 的分析,包括多基基因合成酶 (PKS) 和酶BGCs.
- 抗微生物活性测定和菌株H-BE7.7的化学复制.
主要成果:
- 遗传学分析将Brevibacterium菌株分为四个分类,其中25个菌株被确定为潜在的新物种.
- 泛基因组分析揭示了一个小的核心基因组和大量的辅助基因.
- 观察到多种不同的BGC,其中一些显示分类特定的分布,如 siderophore,carotenoid 和 PKS BGC.
- 菌株H-BE7对Salmonella enterica和Listeria monocytogenes表现出抗菌活性. 这种菌株具有抗菌作用.
- 在H-BE7提取物中鉴定出1-甲基,尽管没有正规的酶生物合成基因,这表明一种未知的途径.
结论:
- 探索多样化的生态系统对于揭示Brevibacterium的全部多样性和识别新型抗生素生产商至关重要.
- 基因组和泛基因组分析为Brevibacterium属的进化动态和生物合成潜力提供了洞察力.
- 菌株H-BE7是进一步研究的有希望的候选人,因为它具有抗菌活性,并存在未表征的1-甲基胺生物合成途径.
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