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在FFPE组织段中进行人类宿主-病原体同位化研究的双空间分辨率转录组学.

Hailey Sounart1, Enikő Lázár1,2, Yuvarani Masarapu1

  • 1Department of Gene Technology, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.

Genome biology
|October 20, 2023
PubMed
概括

本研究引入了一个空间转录学方法,用于分析宿主和病原体基因表达在甲固定嵌 (FFPE) 组织中. 该方法揭示了在肺细胞中感染SARS-CoV-2期间宿主反应的关键见解.

关键词:
定位分析分析的分析.嵌入式甲固定 (FFPE) 组织.主体病原体相互作用空间转录组学 空间转录组学

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科学领域:

  • 传染性疾病 传染性疾病
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 局部化宿主-病原体相互作用对于理解感染动态至关重要,但对研究具有挑战性.
  • 现有的技术往往缺乏分辨率或范围,无法同时分析组织微环境中的宿主和病原体的分子反应.

研究的目的:

  • 开发和验证一种新的空间转录组学方法,用于在FFPE组织段中同时对宿主和病原体进行高分辨率的全转录组分析.
  • 为了研究在人类肺组织中SARS-CoV-2感染期间宿主-病原体相互作用的空间景观.

主要方法:

  • 开发一种空间转录组学技术,使宿主和病原体的同时转录组范围内的基因表达概况成为可能.
  • 该方法应用于来自COVID-19患者的甲固化嵌入式 (FFPE) 人类肺组织样本.
  • 使用RNAScope和现场测序验证空间SARS-CoV-2检测.

主要成果:

  • 该方法成功捕获了宿主和SARS-CoV-2的空间基因表达数据,在FFPE肺组织中接近单细胞分辨率.
  • 空间局部化分析确定了调节SARS-CoV-2感染的潜在宿主因素.
  • 验证证实了病毒空间检测的准确性.

结论:

  • 这种新的空间转录学方法为FFPE组织内的空间层面上宿主和病原体之间的复杂相互作用提供了前所未有的见解.
  • 该技术可促进两种转录组的无偏,同时检测,促进传染病和宿主反应的研究.
  • 这些发现有助于了解宿主对SARS-CoV-2感染的反应,并可能指导治疗策略.