在小鼠中,疫苗介导的防治梅尔贝科病毒和沙尔贝科病毒的挑战
David R Martinez1, Alexandra Schäfer2, Tyler D Gavitt3
1Department of Immunobiology, Yale School of Medicine, New Haven, CT 06510, USA; Yale Center for Infection and Immunity, Yale School of Medicine, New Haven, CT 06510, USA.
Cell reports
|October 20, 2023
概括
一种针对SARS-CoV-2,RSSHC014和MERS-CoV的受体结合域 (RBD) 的新型三价纳米粒子疫苗显示出对小鼠多种致病性冠状病毒的广泛保护.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 疫苗开发 疫苗开发
背景情况:
- 三种高度致病的人类冠状病毒 (SARS-CoV,MERS-CoV,SARS-CoV-2) 已经出现,需要针对Merbecovirus和Sarbecovirus亚系有效的疫苗.
- 目前的SARS-CoV-2疫苗对其他sarbecoviruses和merbecoviruses提供有限的保护.
研究的目的:
- 开发和评估一种广泛的防护疫苗,以对抗Merbecovirus和Sarbecovirus亚种.
- 在临床前模型中评估三价纳米粒子疫苗的疗效.
主要方法:
- 小鼠接种了含有来自SARS-CoV-2,RSSHC014和MERS-CoV的受体结合域 (RBD) 的三价位分类酶结合纳米粒子 (scNP) 疫苗.
- 在致命的小鼠模型中评估了中和抗体反应和对活病毒挑战的保护.
主要成果:
- 三价RBD scNP疫苗引起了对一系列冠状病毒的中和抗体,包括WIV-1-CoV,SARS-CoV,SARS-CoV-2变种和MERS-CoV.
- 受到三价疫苗保护的小鼠对Merbecovirus和Sarbecovirus都具有挑战性,与单价SARS-CoV-2疫苗不同,该疫苗只对Sarbecovirus进行了保护.
结论:
- 一个单一的三价RBD scNP疫苗证明了对三种高度致病的人类冠状病毒的广泛保护的概念证明,涵盖两个贝塔冠状病毒亚种.
- 这种方法为开发泛沙尔贝病毒和泛梅尔贝病毒疫苗提供了潜在的战略.
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