响应pH和宿主-客主活性聚合体的可逆拥挤:模仿微米大小的细胞结构
Kehu Zhang1, Yang Zhou1, Silvia Moreno2
1Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, Dresden 01069, Germany; Chair of Organic Chemistry of Polymers, Technische Universität Dresden, Dresden 01062, Germany.
Journal of colloid and interface science
|October 20, 2023
概括
研究人员开发了一种新的交叉连接方法,从聚合物体中创建集群的人工器官 (AO). 这种技术允许控制聚合和重组,模仿复杂的细胞结构,用于系统生物学应用.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 细胞生物学 细胞生物学
背景情况:
- 孤立的人工器官 (AO) 已知结构-功能关系,但集群的AO仍未得到充分研究.
- 模仿复杂的,异质的细胞结构需要能够进行可逆聚合的刺激响应的体系统.
研究的目的:
- 制定制造具有适应性重组能力的聚合物聚合体的策略.
- 创建一个平台,使用动态,集群的人工器官来建模生物系统.
主要方法:
- 利用了含有阿佐的聚合体 (Azo-Psomes) 和用于交叉链接的β-cyclodextrin修饰聚合物 (β-CD聚合物) 之间的宿主-客相互作用.
- 使用化学刺激 (crosslinker/decrosslinker) 和环境pH来控制可逆 (拆卸) 和集群大小.
- 包含一个温度敏感的交联剂,用于温度介导的凝聚.
主要成果:
- 通过使用特定的交叉链接器和断层链接器,证明了聚类阿佐-体的可逆 (分解) 组装.
- 展示了pH取决于集群大小的控制和同质/异质的Azo-Psomes结构的制造.
- 通过光酶级联反应实现了聚类AO的可视化,并证明了温度触发的凝聚.
结论:
- 开发的交叉连接策略使集群人工有机体的控制制造和动态重组成为可能.
- 这些可适应的聚合聚合体为建模生物系统和推进系统生物学研究提供了一个有前途的平台.
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