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Updated: Jul 12, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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在单核酸分辨率下,IsoSeek从低输入样本中对miRNA进行无偏见和UMI信息的测序

Monique A J van Eijndhoven1, Chantal Scheepbouwer2, Ernesto Aparicio-Puerta3

  • 1Amsterdam UMC, Location Vrije Universiteit Amsterdam, Department of Pathology, 1081 HV Amsterdam, the Netherlands; Cancer Center Amsterdam, Imaging and Biomarkers, 1081 HV Amsterdam, the Netherlands.

STAR protocols
|October 20, 2023
PubMed
概括

IsoSeek 是一种用于准确检测 isomiRs 的新协议, isomiRs 是重要的微RNA (miRNA) 变异. 这种方法通过减少测序偏差来提高检测准确度,特别是对于低输入样本.

关键词:
生物信息学是一种生物信息学.生物技术和生物工程 生物技术和生物工程遗传学 遗传学 是一个基因组学就是基因组学.

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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